Anti-Tubulin beta Monoclonal Antibody (OAAI00280)

• Product Number: OAAI00280
• Name: Anti-Tubulin beta Monoclonal Antibody (OAAI00280)
• Molecular Weight: 55
• Clone: ABM323
• Isotype: IgG1
• NCBI Gene Id: 203068
• Host: Mouse
• Clonality: Monoclonal
• Concentration: 100ug/200ul
• Gene Full Name: tubulin beta class I
• Alias Symbols: beta Ib tubulin;CDCBM6;CSCSC1;epididymis secretory sperm binding protein;M40;OK/SW-cl.56;TUBB1;TUBB5;tubulin beta chain;tubulin beta-1 chain;tubulin beta-5 chain;tubulin, beta polypeptide.
• Product Format: Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Reference: Wang, D., Villasante, A et al. The mammalian beta-tubulin repertoire: hematopoietic expression of a novel, heterologous beta-tubulin isotype. J. Cell Biol. 103 (5), 1903-1910 (1986).
  
• Description of Target: Tubulin is the major building block of microtubules which is essential in cell structures, transportation and mitosis. This superfamily is composed of six distinct families. Alpha, beta, and gamma tubulin families are found in all eukaryotes.
  The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The abundance of Gamma tubulin is less than 1% of the level of either alpha or beta tubulin. It shares approximately 28-32% identity with alpha tubulin from various organisms and 32-36% identity with beta tubulins. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton.
  
• Reconstitution and Storage: The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
• Specificity: TUBB
• Additional Information: Background: Tubulin is the major building block of microtubules which is essential in cell structures, transportation and mitosis. This superfamily is composed of six distinct families. Alpha, beta, and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The abundance of Gamma tubulin is less than 1% of the level of either alpha or beta tubulin. It shares approximately 28-32% identity with alpha tubulin from various organisms and 32-36% identity with beta tubulins. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton.
• Additional Information: Positive Control: Lung tissue, Hela cell
• Application Info: WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested
• Immunogen: A synthetic peptide derived from Internal sequence of Tubulin beta protein. This sequence is identical to human, mouse, rat, chicken and dog species.
• Additional Information: Cellular Location: Cytoplasmic
• Formulation: This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Uniprot ID: P07437
• Purification: The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping
• Gene Symbol: TUBB
• Predicted Species Reactivity: Human|Mouse|Rat
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot
• Image 1: A431, Hela
  
  WB: Cell lysate from A431 (lane1) and Hela (Lane 2) was resolved onto 12% SDS -PAGE and transferred onto NC Membrane, then probed by Mouse anti -Tubulin beta antibody, (Cat#OAAI00280) at 1:500. An immune -reactive band ~55 kDa is observed.
• Image 2: Human Lung
  
  IHC: Human lung tissue stained with Mouse anti -Tubulin-beta antibody, (Cat#OAAI00280) at 1:25 for 10 min @ RT. Staining of formalin -fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.