| Product Number |
OAAH00068 |
| Product Page |
www.avivasysbio.com/hist3h3-antibody-oaah00068.html |
| Name |
HIST3H3 Antibody (OAAH00068) |
| Protein Size (# AA) |
136 amino acids |
| Molecular Weight |
16 kDa |
| Isotype |
Rabbit lgG |
| NCBI Gene Id |
8290 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Source |
Rabbit pAb |
| Gene Full Name |
Histone H3.1t |
| Alias Symbols |
H3t, H3.4, H3/g, H3FT, HIST3H3 |
| Reference |
1. Albig, W., Doenecke, D., Hum. Genet. 101: 284-294, 1997.
2. Albig, W., Ebentheuer, J., Klobeck, G., Hum. Genet. 97: 486-491, 1996.
3. Yamagishi, Y., Honda, T., Tanno, Y., Science 330: 239-233, 2010.
|
| Description of Target |
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. |
| Reconstitution and Storage |
Store the product at -20C. Stable for one year from the date of shipment. |
| Datasheets/Manuals |
Printable datasheet for anti-HIST3H3 (OAAH00068) antibody |
| Specificity |
H3K4me3 polyclonal antibody detects endogenous levels of Histone H3 tri-methylation at Lys4. |
| Additional Information |
Localization: Nucleus |
| Application Info |
WB: 1:1000-1:4000
DB: 1:500
|
| Immunogen |
This Aviva H3K4me3 polyclonal antibody was produced by immunizing rabbits with synthetic Histone H3 peptide with Lys4 tri-methylated and conjugated to KLH. |
| Uniprot ID |
Q16695 |
| Protein Name |
Histone H3.1t |
| Protein Accession # |
NP_003484.1 |
| Nucleotide Accession # |
NM_003493.2 |
| Gene Symbol |
HIST3H3 |
| Application |
WB, DB, CHIP |
| Image 1 | HCT116
 | Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.
|
| | Image 2 | 293T nuclear extract
 | Endogenous H3K4me3 detected in 293T nuclear extract
Primary antibody: 1:1000 |
| | Image 3 | H3K4me3 peptide
 | This antibody was tested on a peptide-chip (peptides loaded shown in the left panel) by dot blot assay, which showed a specific reaction with H3K4me3peptide (right panel). me: methylated; ac: acetylated; as: asymmetric
Primary antibody: 1:500 |
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