HIST3H3 Antibody (OAAH00059)

Data Sheet
 
Product Number OAAH00059
Product Page www.avivasysbio.com/hist3h3-antibody-oaah00059.html
Name HIST3H3 Antibody (OAAH00059)
Protein Size (# AA) 136 amino acids
Molecular Weight 16 kDa
Isotype Rabbit lgG
NCBI Gene Id 8290
Host Rabbit
Clonality Polyclonal
Source Rabbit pAb
Gene Full Name Histone H3.1t
Alias Symbols H3t, H3.4, H3/g, H3FT, HIST3H3
Reference 1. Benhar, M., Forrester, M. T., Hess, D. T., Science 320: 1050-1054, 2008.
2. Gervais, F. G., Xu, D., Robertson, G. S., Cell 97: 395-406, 1999.
3. Lakhani, S. A., Masud, A., Kuida, K., Science 311: 847-851, 2006
Description of Target Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family.
Reconstitution and Storage Store the product at -20C. Stable for one year from the date of shipment.
Datasheets/Manuals Printable datasheet for anti-HIST3H3 (OAAH00059) antibody
Specificity H3K79me2 polyclonal antibody detects endogenous levels of Histone H3 di- methylated at Lys79.
Additional Information Localization: Nucleus
Application Info WB: 1:1000-1:4000
IF: 1:100-1:500
DB: 1:500
Immunogen This Aviva H3K79me2 polyclonal antibody was produced by immunizing rabbits with synthetic Histone H3 peptide with Lys79 di-methylated and conjugated to KLH.
Uniprot ID Q16695
Protein Name Histone H3.1t
Protein Accession # NP_003484.1
Nucleotide Accession # NM_003493.2
Gene Symbol HIST3H3
Application WB, IF, DB, CHIP
Image 1
H3K79me2 peptide
This antibody was tested on a peptide-chip (peptides loaded shown in the left panel)
by dot blot assay, which showed a specific reaction with H3K79me2 peptide (right panel). me: methylated; ac: acetylated; as: asymmetric
Primary antibody: 1:500
Image 2
HCT116
Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.
Image 3
293T
IF analysis of H3K79me2 in 293T cells. Green: anti-H3K79me2; Blue: DAPI
Primary antibody:1:500
Image 4
293T nuclear extract
Endogenous H3K79me2 detected in 293T nuclear extract
Primary antibody: 1:1000
 

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