Product Number |
OAAH00059 |
Product Page |
www.avivasysbio.com/hist3h3-antibody-oaah00059.html |
Name |
HIST3H3 Antibody (OAAH00059) |
Protein Size (# AA) |
136 amino acids |
Molecular Weight |
16 kDa |
Isotype |
Rabbit lgG |
NCBI Gene Id |
8290 |
Host |
Rabbit |
Clonality |
Polyclonal |
Source |
Rabbit pAb |
Gene Full Name |
Histone H3.1t |
Alias Symbols |
H3t, H3.4, H3/g, H3FT, HIST3H3 |
Reference |
1. Benhar, M., Forrester, M. T., Hess, D. T., Science 320: 1050-1054, 2008. 2. Gervais, F. G., Xu, D., Robertson, G. S., Cell 97: 395-406, 1999. 3. Lakhani, S. A., Masud, A., Kuida, K., Science 311: 847-851, 2006
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Description of Target |
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. |
Reconstitution and Storage |
Store the product at -20C. Stable for one year from the date of shipment. |
Datasheets/Manuals |
Printable datasheet for anti-HIST3H3 (OAAH00059) antibody |
Specificity |
H3K79me2 polyclonal antibody detects endogenous levels of Histone H3 di- methylated at Lys79. |
Additional Information |
Localization: Nucleus |
Application Info |
WB: 1:1000-1:4000 IF: 1:100-1:500 DB: 1:500
|
Immunogen |
This Aviva H3K79me2 polyclonal antibody was produced by immunizing rabbits with synthetic Histone H3 peptide with Lys79 di-methylated and conjugated to KLH. |
Uniprot ID |
Q16695 |
Protein Name |
Histone H3.1t |
Protein Accession # |
NP_003484.1 |
Nucleotide Accession # |
NM_003493.2 |
Gene Symbol |
HIST3H3 |
Application |
WB, IF, DB, CHIP |
Image 1 | H3K79me2 peptide
| This antibody was tested on a peptide-chip (peptides loaded shown in the left panel) by dot blot assay, which showed a specific reaction with H3K79me2 peptide (right panel). me: methylated; ac: acetylated; as: asymmetric
Primary antibody: 1:500 |
| Image 2 | HCT116
| Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.
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| Image 3 | 293T
| IF analysis of H3K79me2 in 293T cells. Green: anti-H3K79me2; Blue: DAPI
Primary antibody:1:500 |
| Image 4 | 293T nuclear extract
| Endogenous H3K79me2 detected in 293T nuclear extract
Primary antibody: 1:1000 |
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