HIST1H4A Antibody (OAAH00057)

• Product Number: OAAH00057
• Name: HIST1H4A Antibody (OAAH00057)
• Protein Size (# AA): 103 amino acids
• Molecular Weight: 11 kDa
• Isotype: Rabbit lgG
• NCBI Gene Id: 121504; 554313; 8294; 8359; 8360; 8361; 8362; 8363; 8364; 8365; 8366; 8367; 8368; 8370;
• Host: Rabbit
• Clonality: Polyclonal
• Source: Rabbit pAb
• Gene Full Name: Histone H4
• Alias Symbols: H4/p, H4C1, H4C2, H4C3, H4C4, H4C5, H4C6, H4C8, H4C9, H4C11, H4C12, H4C13, H4C14, H4C15, HIST4H4
• Reference: 1. Fraga MF, Ballestar E, Villar-Garea A, Nat Genet. 2005 ; 391-400.
  2. Braastad CD, Hovhannisyan H, van Wijnen AJ. Gene. 2004 : 35-40.
  3. Marzluff WF, Gongidi P, Woods KR, Genomics. 2002 : 487-98.
  
• Description of Target: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family.
• Reconstitution and Storage: Store the product at -20C. Stable for one year from the date of shipment.
• Specificity: H4K20me1 polyclonal antibody detects endogenous levels of Histone H4 mono-methylated at Lys20.
• Additional Information: Localization: Nucleus
• Application Info: WB: 1:1000-1:4000
  IF: 1:100-1:500
  DB: 1:500
  
• Immunogen: This Aviva H4K20me1 polyclonal antibody was produced by immunizing rabbits with synthetic Histone H4 peptide with Lys20 mono-methylated and conjugated to KLH.
• Uniprot ID: P62805
• Protein Name: Histone H4
• Protein Accession #: NP_001029249.1; NP_003486.1; NP_003529.1; NP_003530.1; NP_003531.1; NP_003532.1; NP_003533.1; NP_003534.1; NP_003535.1
• Nucleotide Accession #: NM_001034077.4; NM_003495.2; NM_003538.3; NM_003539.3; NM_003540.3; NM_003541.2; NM_003542.3; NM_003543.3; NM_003544.2
• Gene Symbol: HIST1H4A
• Application: WB, IF, DB, CHIP
• Image 1: 293T nuclear extract
  
  This antibody was tested on a peptide-chip (peptides loaded shown in the left panel) by dot blot assay, which showed a specific reaction with H4K20me1 peptide (right panel). me: methylated; ac: acetylated; as: asymmetric
  Primary antibody: 1:500
• Image 2: HCT116
  
  Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.
• Image 3: HCT116
  
  Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.
• Image 4: H4K20me1 peptide
  
  IF analysis of H4K20me1 in 293T cells. Green: anti-H4K20me1; Blue: DAPI
  Primary antibody: 1:500