Lck Antibody (Phospho-Tyr505) (OAAF07396)

• Product Number: OAAF07396
• Name: Lck Antibody (Phospho-Tyr505) (OAAF07396)
• Molecular Weight: 58 kDa
• Isotype: IgG
• NCBI Gene Id: 3932
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Gene Full Name: LCK proto-oncogene, Src family tyrosine kinase
• Alias Symbols: IMD22;leukocyte C-terminal Src kinase;LSK;lymphocyte cell-specific protein-tyrosine kinase;p56(LSTRA) protein-tyrosine kinase;p56lck;p56-LCK;pp58lck;Protein YT16;Proto-oncogene Lck;proto-oncogene tyrosine-protein kinase LCK;t cell-specific protein-tyrosine kinase;T-lymphocyte specific protein tyrosine kinase p56lck;tyrosine-protein kinase Lck;YT16.
• Product Format: Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Description of Target: Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosine residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK also contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP. Interacts with FYB2 (PubMed:27335501).
• Reconstitution and Storage: -20°C
• Specificity: Lck (Phospho-Tyr505) Antibody detects endogenous levels of Lck only when phosphorylated at Tyr505.
• Additional Information: Modification Sites: H:Y505, M:Y505, R:Y505
• Application Info: WB 1:500~1000
  IHC 1:50~100
  ELISA 1:60000
  Optimal dilutions should be determined by the end user.
• Immunogen: The antiserum was produced against synthesized peptide derived from human Lck around the phosphorylation site of Tyr505.
• Intended Use: This product is for research use only, not for use in diagnostic or therapeutic procedures.
• Uniprot ID: P06239
• Protein Name: Tyrosine-protein kinase Lck
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Target Post-Translational Modification: Phospho
• Gene Symbol: LCK
• Predicted Species Reactivity: Human|Mouse|Rat
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Image 1: Paraffin-embedded human breast carcinoma
  
  Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Lck (Phospho-Tyr505) Antibody. The picture on the right is blocked with the phospho peptide.
• Image 2: Jurkat cells
  
  Western blot analysis of lysates from Jurkat cells, using Lck (Phospho-Tyr505) Antibody. The lane on the right is blocked with the phospho peptide.