MAP2K7 Antibody (Phospho-Ser271) (OAAF05547)

Data Sheet
 
Product Number OAAF05547
Product Page www.avivasysbio.com/map2k7-antibody-phospho-ser271-oaaf05547.html
Name MAP2K7 Antibody (Phospho-Ser271) (OAAF05547)
Molecular Weight 47 kDa
Isotype IgG
NCBI Gene Id 5609
Host Rabbit
Clonality Polyclonal
Concentration 1 mg/ml
Description
Alias Symbols MEK, MKK7, JNKK2, MEK 7, MAPKK7, PRKMK7, SAPKK4, SAPKK-4
Product Format LiquidPBS (without Mg2+ and Ca2+) (pH 7.4), 150mM NaCl, 0.02% sodium azide and 50% glycerol
Reconstitution and Storage Store at -20°C/1 year
Datasheets/Manuals Printable datasheet for OAAF05547
Specificity MAP2K7 (Phospho-Ser271) Antibody detects endogenous levels of MAP2K7 only when phosphorylated at S271
Additional Information Modification Sites: Human: S271, Mouse: S287, Rat: S271
Immunogen The antiserum was produced against synthesized phospho-peptide derived from the Internal region of human MAP2K7 around the phosphorylation site of S271.
Uniprot ID O14733
Publications

Hepatocyte TRAF3 promotes liver steatosis and systemic insulin resistance through targeting TAK1-dependent signalling. Nat Commun. 7, 10592 (2016). 26882989

Targeting CASP8 and FADD-like apoptosis regulator ameliorates nonalcoholic steatohepatitis in mice and nonhuman primates. Nat. Med. 23, 439-449 (2017). 28218919

Purification The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Target Post-Translational Modification Phospho
Gene Symbol MAP2K7
Predicted Species Reactivity Human, Mouse, Rat
Application WB, IHC, ELISA
Image 1
NIH-3T3 cells
Western blot analysis of extracts from NIH-3T3 cells treated with insulin 0.01U/ml 15' and NIH-3T3 cells treated with EGF 200ng/ml 30', using MAP2K7 (Phospho-Ser271) Antibody.
Image 2
MAP2K7 (Phospho-Ser271)
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MAP2K7 (Phospho-Ser271) Antibody
 

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