MAP2K7 Antibody (Phospho-Ser271) (OAAF05547)

Data Sheet
Product Number OAAF05547
Product Page
Name MAP2K7 Antibody (Phospho-Ser271) (OAAF05547)
Gene Symbol MAP2K7
Alias Symbols c-Jun N-terminal kinase kinase 2, Dual specificity mitogen-activated protein kinase kinase 7, JNK activating kinase 2, JNK kinase 2, JNKK 2, JNKK2, kinase MKK7, MAP kinase kinase 7, MAP2K7, MAPK/ERK kinase 7, MAPKK 7, MP2K7, PRKMK7
Molecular Weight 47 kDa
Product Format LiquidPBS (without Mg2+ and Ca2+) (pH 7.4), 150mM NaCl, 0.02% sodium azide and 50% glycerol
Isotype IgG
NCBI Gene Id 5609
Host Rabbit
Clonality Polyclonal
Concentration 1 mg/ml
Reconstitution and Storage Store at -20C/1 year
Datasheets/Manuals Printable datasheet for OAAF05547
Specificity MAP2K7 (Phospho-Ser271) Antibody detects endogenous levels of MAP2K7 only when phosphorylated at S271
Additional Information Modification Sites: Human: S271, Mouse: S287, Rat: S271
Application Info WB: 1:500~1000
IHC: 1:50~100
ELISA: 1:20000
Immunogen The antiserum was produced against synthesized phospho-peptide derived from the Internal region of human MAP2K7 around the phosphorylation site of S271. Loc: 236-285aa
Swissprot Id O14733

Hepatocyte TRAF3 promotes liver steatosis and systemic insulin resistance through targeting TAK1-dependent signalling. Nat Commun. 7, 10592 (2016). WB, IHC, ELISA, Human, Mouse, Rat26882989

Targeting CASP8 and FADD-like apoptosis regulator ameliorates nonalcoholic steatohepatitis in mice and nonhuman primates. Nat. Med. 23, 439-449 (2017). WB, IHC, ELISA, Human, Mouse, Rat28218919

Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phospho peptide.
Target Post-Translational Modification Phospho
Replacement Item This antibody may replace item sc-136337 from Santa Cruz Biotechnology.
Predicted Species Reactivity Human, Mouse, Rat
Application WB, IHC
Image 1
NIH-3T3 cells
Western blot analysis of extracts from NIH-3T3 cells treated with insulin 0.01U/ml 15' and NIH-3T3 cells treated with EGF 200ng/ml 30', using MAP2K7 (Phospho-Ser271) Antibody.
Image 2
MAP2K7 (Phospho-Ser271)
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MAP2K7 (Phospho-Ser271) Antibody

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