| Product Number |
OAAF05409 |
| Product Page |
www.avivasysbio.com/ack-antibody-phospho-tyr518-oaaf05409.html |
| Name |
ACK Antibody (Phospho-Tyr518) (OAAF05409) |
| Molecular Weight |
114 kDa |
| NCBI Gene Id |
10188 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1 mg/ml |
| Alias Symbols |
ACK, ACK1, ACK-1, p21cdc42Hs |
| Reconstitution and Storage |
Store at -20C/1 year |
| Datasheets/Manuals |
Printable datasheet for OAAF05409 |
| Specificity |
ACK (Phospho-Tyr518) antibody detects endogenous levels of ACK only when phosphorylated at Tyr518. |
| Additional Information |
Modification Sites: Human: Y518, Rat: Y518 |
| Application Info |
WB: 1:500 - 1:1000
ELISA: 1:10000 |
| Immunogen |
The antiserum was produced against synthesized phospho-peptide derived from the Internal region of human ACK around the phosphorylation site of Tyr518. |
| Formulation |
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Uniprot ID |
Q07912 |
| Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
| Gene Symbol |
TNK2 |
| Predicted Species Reactivity |
Human, Rat |
| Application |
WB |
| Image 1 | HepG2 cells
 | Western blot analysis of extracts from HepG2 cells treated with EGF, using phospho-ACK (Phospho-Tyr518) antibody. |
| | Image 2 | ACK (Phospho-Tyr518)
 | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using ACK (Phospho-Tyr518) Antibody |
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