| Product Number |
OAAF02774 |
| Product Page |
www.avivasysbio.com/atg4b-antibody-oaaf02774.html |
| Name |
ATG4B Antibody (OAAF02774) |
| Molecular Weight |
44 kDa |
| NCBI Gene Id |
23192 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Gene Full Name |
autophagy related 4B cysteine peptidase |
| Alias Symbols |
APG4 autophagy 4 homolog B;APG4B;ATG4 autophagy related 4 homolog B;AUTL1;AUT-like 1 cysteine endopeptidase;autophagin-1;autophagy-related cysteine endopeptidase 1;autophagy-related protein 4 homolog B;cysteine protease ATG4B;hAPG4B. |
| Product Format |
Liquid |
| Reference |
Kabeya Y., J. Cell Sci. 117:2805-2812(2004).
Marino G., J. Biol. Chem. 278:3671-3678(2003).
Ota T.,Nat. Genet. 36:40-45(2004). |
| Description of Target |
Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms. |
| Reconstitution and Storage |
-20°C |
| Datasheets/Manuals |
Printable datasheet for ATG4B Antibody (OAAF02774) |
| Specificity |
ATG4B antibody detects endogenous levels of total ATG4B protein. |
| Application Info |
WB: 1:500~1:3000; IHC: 1:50~1:100; ELISA: 1:20000 |
| Immunogen |
The antiserum was produced against synthesized peptide derived from human ATG4B. |
| Dilution |
The optimal working dilution should be determined by the end user. |
| Formulation |
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Uniprot ID |
Q9Y4P1 |
| Protein Name |
Cysteine protease ATG4B |
| Publications |
NKCT1 (purified Naja kaouthia protein toxin) conjugated gold nanoparticles induced Akt/mTOR inactivation mediated autophagic and caspase 3 activated apoptotic cell death in leukemic cell. Toxicon. 121, 86-97 (2016). 27527270 |
| Protein Accession # |
NP_037457.3 |
| Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Gene Symbol |
ATG4B |
| Predicted Species Reactivity |
Human|Mouse |
| Application |
Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot |
| Image 1 | human skeletal muscle tissue
 | Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using ATG4B Antibody. The picture on the right is treated with the synthesized peptide. |
|
| Image 2 | Jurkat cells
 | Western blot analysis of extracts from Jurkat cells, using ATG4B Antibody. The lane on the right is treated with the synthesized peptide. |
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