EP300 Antibody (OAAF01891)

• Product Number: OAAF01891
• Name: EP300 Antibody (OAAF01891)
• Molecular Weight: 264 kDa
• Isotype: IgG
• NCBI Gene Id: 2033
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Gene Full Name: E1A binding protein p300
• Description:
  View product citations for EP300 Antibody (OAAF01891) on CiteAb
• Alias Symbols: E1A-associated protein p300;E1A-binding protein, 300kD;histone acetyltransferase p300;histone butyryltransferase p300;histone crotonyltransferase p300;KAT3B;MKHK2;p300;p300 HAT;protein 2-hydroxyisobutyryltransferase p300;protein lactyltransferas p300;protein propionyltransferase p300;RSTS2.
• Product Format: Liquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
• Reference: Wuchao Yuan, J. Biol. Chem., Apr 1996; 271: 9009.
  E. Tory Manning, Mol. Cell. Biol., Jun 2001; 21: 3876 - 3887.
  Jiwen Li, Mol. Cell. Biol., Mar 2000; 20: 2031 - 2042.
  Gopalswamy Jayaraman, J. Biol. Chem., Jun 1999; 274: 17342.
• Description of Target: Functions as histone acetyltransferase and regulates transcription via chromatin remodeling (PubMed:23415232, PubMed:23934153, PubMed:8945521). Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation (PubMed:23415232, PubMed:23934153, PubMed:8945521). Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Mediates acetylation of histone H3 at 'Lys-122' (H3K122ac), a modification that localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability. Mediates acetylation of histone H3 at 'Lys-27' (H3K27ac) (PubMed:23911289). Also functions as acetyltransferase for nonhistone targets. Acetylates 'Lys-131' of ALX1 and acts as its coactivator (PubMed:12929931). Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function (PubMed:18722353). Acetylates HDAC1 leading to its inactivation and modulation of transcription (PubMed:16762839). Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2 (PubMed:12586840). Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium. Promotes cardiac myocyte enlargement. Can also mediate transcriptional repression. Acetylates FOXO1 and enhances its transcriptional activity (PubMed:15890677). Acetylates BCL6 wich disrupts its ability to recruit histone deacetylases and hinders its transcriptional repressor activity (PubMed:12402037). Participates in CLOCK or NPAS2-regulated rhythmic gene transcription; exhibits a circadian association with CLOCK or NPAS2, correlating with increase in PER1/2 mRNA and histone H3 acetylation on the PER1/2 promoter (PubMed:14645221). Acetylates MTA1 at 'Lys-626' which is essential for its transcriptional coactivator activity (PubMed:16617102). Acetylates XBP1 isoform 2; acetylation increases protein stability of XBP1 isoform 2 and enhances its transcriptional activity (PubMed:20955178). Acetylates PCNA; acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER) (PubMed:24939902). Acetylates MEF2D (PubMed:21030595). Acetylates and stabilizes ZBTB7B protein by antagonizing ubiquitin conjugation and degragation, this mechanism may be involved in CD4/CD8 lineage differentiation (PubMed:20810990). In addition to protein acetyltransferase, can use different acyl-CoA substrates, such as (2E)-butenoyl-CoA (crotonyl-CoA), butanoyl-CoA (butyryl-CoA) or propanoyl-CoA (propionyl-CoA), and is able to mediate protein crotonylation, butyrylation or propionylation, respectively (PubMed:25818647, PubMed:17267393). Acts as a histone crotonyltransferase; crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors (PubMed:25818647). Histone crotonyltransferase activity is dependent on the concentration of (2E)-butenoyl-CoA (crotonyl-CoA) substrate and such activity is weak when (E)-but-2-enoyl-CoA (crotonyl-CoA) concentration is low (PubMed:25818647). Also acts as a histone butyryltransferase; butyrylation marks active promoters (PubMed:17267393).
• Reconstitution and Storage: -20°C
• Specificity: p300 antibody detects endogenous levels of total p300 protein.
• Application Info: WB: 1/500 - 1/2000
  IHC: 1/100 - 1/300
  ELISA: 1/10000
• Immunogen: The antiserum was produced against synthesized peptide derived from human p300.
• Uniprot ID: Q09472
• Protein Name: Histone acetyltransferase p300
• Publications:

  Differential Kat3 Usage Orchestrates the Integration of Cellular Metabolism with Differentiation. Cancers (Basel). 13 (2021). 34884992

• Protein Accession #: NP_001420.2
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using epitope specific immunogen.
• Gene Symbol: EP300
• Predicted Species Reactivity: Human|Mouse|Rat
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Image 1: MDA-MB-435 cells
  
  Western blot analysis of extracts from MDA-MB-435 cells, using p300 Antibody. The lane on the right is blocked with the synthesized peptide.
• Image 2: Human Lung Carcinoma Tissue
  
  Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using p300 Antibody. The picture on the right is blocked with the synthesized peptide.