GZMB Antibody (OAAF01817)

Data Sheet
 
Product Number OAAF01817
Product Page www.avivasysbio.com/gzmb-antibody-oaaf01817.html
Name GZMB Antibody (OAAF01817)
Molecular Weight 27 kDa
Isotype IgG
NCBI Gene Id 2999|3002
Host Rabbit
Clonality Polyclonal
Concentration 1 mg/ml
Gene Full Name granzyme B|granzyme H
Alias Symbols C11;cathepsin G-like 1;Cathepsin G-like 2;cathepsin G-like 2, protein h-CCPX;CCPI;CCP-X;CGL1;CGL-1;CGL-2;CSPB;CSP-B;CSP-C;CTLA1;CTLA-1;CTSGL1;CTSGL2;cytotoxic serine protease B;Cytotoxic serine protease C;cytotoxic T-lymphocyte proteinase;cytotoxic T-lymphocyte proteinase 2;cytotoxic T-lymphocyte-associated serine esterase 1;cytotoxin serine protease-C;fragmentin 2;Fragmentin-2;granzyme B;granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1);granzyme H;Granzyme-2;HLP;human lymphocyte protein;SECT;T-cell serine protease 1-3E.
Product Format Liquid PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Reference David A. Jans, J. Biol. Chem., Nov 1996; 271: 30781.
Eugene Estella, Diabetes, Aug 2006; 55: 2212 - 2219.
Yong Gu, J. Biol. Chem., May 1996; 271: 10816.
Jiuru Sun, J. Biol. Chem., Nov 1996; 271: 27802.
Description of Target Cytotoxic chymotrypsin-like serine protease with preference for bulky and aromatic residues at the P1 position and acidic residues at the P3' and P4' sites. Probably necessary for target cell lysis in cell-mediated immune responses. Participates in the antiviral response via direct cleavage of several proteins essential for viral replication.|This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
Reconstitution and Storage -20°C
Datasheets/Manuals Printable datasheet for GZMB Antibody (OAAF01817)
Specificity Granzyme B antibody detects endogenous levels of total Granzyme B protein.
Application Info WB: 1:500~1000
IHC: 1:50~100
ELISA: 1:5000
Immunogen The antiserum was produced against synthesized peptide derived from human Granzyme B.
Dilution The optimal working dilution should be determined by the end user.
Uniprot ID P10144|P20718
Protein Name Granzyme B|Granzyme H
Publications

Influenza A surface glycosylation and vaccine design. Proc. Natl. Acad. Sci. U.S.A. 114, 280-285 (2017). 28028222

Protein Accession # NP_004122.2
Purification The antibody was purified from rabbit antiserum by affinity chromatography using immunogen.
Gene Symbol GZMB|GZMH
Predicted Species Reactivity Human|Mouse|Rat
Application Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
Image 1
NIH-3T3 cells
Western blot analysis of extracts from NIH-3T3 cells, using Granzyme B Antibody. The lane on the right is treated with the synthesized peptide.
Image 2
human breast carcinoma tissue
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Granzyme B Antibody. The picture on the right is treated with the synthesized peptide.
 

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