FHIT Antibody (OAAF01795)

Data Sheet
 
Product Number OAAF01795
Product Page www.avivasysbio.com/fhit-antibody-oaaf01795.html
Name FHIT Antibody (OAAF01795)
Molecular Weight 16 kDa
NCBI Gene Id 2272
Host Rabbit
Clonality Polyclonal
Gene Full Name fragile histidine triad diadenosine triphosphatase
Alias Symbols AP3A hydrolase;AP3Aase;bis(5'-adenosyl)-triphosphatase;diadenosine 5',5'''-P1,P3-triphosphate hydrolase;dinucleosidetriphosphatase;FRA3B;Fragile histidine triad protein.
Product Format Liquid
Reference Hideshi Ishii, Cancer Res., Dec 2006; 66: 11287 - 11292.
Francesca Bianchi, PNAS, Dec 2006; 103: 18981 - 18986.
Koshi Mimori, Cancer Res., Mar 2006; 66: 2683 - 2690.
Tamotsu Kuroki, Cancer Res., Jul 2003; 63: 3724 - 3728.
Description of Target Cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P(1)-P(4)-bis(5'-adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP. Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5. Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways. Inhibits MDM2-mediated proteasomal degradation of p53/TP53 and thereby plays a role in p53/TP53-mediated apoptosis. Induction of apoptosis depends on the ability of FHIT to bind P(1)-P(3)-bis(5'-adenosyl) triphosphate or related compounds, but does not require its catalytic activity, it may in part come from the mitochondrial form, which sensitizes the low-affinity Ca(2+) transporters, enhancing mitochondrial calcium uptake. Functions as tumor suppressor.
Reconstitution and Storage -20°C
Datasheets/Manuals Printable datasheet for FHIT Antibody (OAAF01795)
Specificity FHIT antibody detects endogenous levels of total FHIT protein.
Application Info WB: 1:500~1:3000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:10000
Immunogen The antiserum was produced against synthesized peptide derived from human FHIT.
Dilution The optimal working dilution should be determined by the end user.
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Uniprot ID P49789
Protein Name Bis(5'-adenosyl)-triphosphatase
Protein Accession # NP_001159715.1
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Gene Symbol FHIT
Predicted Species Reactivity Human|Mouse|Rat
Application Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
Image 1
A549 cells
Western blot analysis of extracts from A549 cells, using FHIT Antibody. The lane on the right is treated with the synthesized peptide.
Image 2
human breast carcinoma tissue
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FHIT Antibody. The picture on the right is treated with the synthesized peptide.
Image 3
A549 cells
Immunofluorescence analysis of A549 cells, using FHIT Antibody. The picture on the right is treated with the synthesized peptide.
 

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