Product Number |
OAAF01795 |
Product Page |
www.avivasysbio.com/fhit-antibody-oaaf01795.html |
Name |
FHIT Antibody (OAAF01795) |
Molecular Weight |
16 kDa |
NCBI Gene Id |
2272 |
Host |
Rabbit |
Clonality |
Polyclonal |
Gene Full Name |
fragile histidine triad diadenosine triphosphatase |
Alias Symbols |
AP3A hydrolase;AP3Aase;bis(5'-adenosyl)-triphosphatase;diadenosine 5',5'''-P1,P3-triphosphate hydrolase;dinucleosidetriphosphatase;FRA3B;Fragile histidine triad protein. |
Product Format |
Liquid |
Reference |
Hideshi Ishii, Cancer Res., Dec 2006; 66: 11287 - 11292. Francesca Bianchi, PNAS, Dec 2006; 103: 18981 - 18986. Koshi Mimori, Cancer Res., Mar 2006; 66: 2683 - 2690. Tamotsu Kuroki, Cancer Res., Jul 2003; 63: 3724 - 3728. |
Description of Target |
Cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P(1)-P(4)-bis(5'-adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP. Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5. Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways. Inhibits MDM2-mediated proteasomal degradation of p53/TP53 and thereby plays a role in p53/TP53-mediated apoptosis. Induction of apoptosis depends on the ability of FHIT to bind P(1)-P(3)-bis(5'-adenosyl) triphosphate or related compounds, but does not require its catalytic activity, it may in part come from the mitochondrial form, which sensitizes the low-affinity Ca(2+) transporters, enhancing mitochondrial calcium uptake. Functions as tumor suppressor. |
Reconstitution and Storage |
-20°C |
Datasheets/Manuals |
Printable datasheet for FHIT Antibody (OAAF01795) |
Specificity |
FHIT antibody detects endogenous levels of total FHIT protein. |
Application Info |
WB: 1:500~1:3000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:10000 |
Immunogen |
The antiserum was produced against synthesized peptide derived from human FHIT. |
Dilution |
The optimal working dilution should be determined by the end user. |
Formulation |
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Uniprot ID |
P49789 |
Protein Name |
Bis(5'-adenosyl)-triphosphatase |
Protein Accession # |
NP_001159715.1 |
Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Gene Symbol |
FHIT |
Predicted Species Reactivity |
Human|Mouse|Rat |
Application |
Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot |
Image 1 | A549 cells
| Western blot analysis of extracts from A549 cells, using FHIT Antibody. The lane on the right is treated with the synthesized peptide. |
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Image 2 | human breast carcinoma tissue
| Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FHIT Antibody. The picture on the right is treated with the synthesized peptide. |
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Image 3 | A549 cells
| Immunofluorescence analysis of A549 cells, using FHIT Antibody. The picture on the right is treated with the synthesized peptide. |
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