NFKB2 Antibody (OAAF01383)

• Product Number: OAAF01383
• Name: NFKB2 Antibody (OAAF01383)
• Molecular Weight: 96 kDa
• NCBI Gene Id: 4791
• Host: Rabbit
• Clonality: Polyclonal
• Gene Full Name: nuclear factor kappa B subunit 2
• Alias Symbols: CVID10;DNA-binding factor KBF2;H2TF1;lymphocyte translocation chromosome 10 protein;LYT10;LYT-10;NFKB, p52/p100 subunit;NF-kB2;nuclear factor Kappa-B, subunit 2;nuclear factor NF-kappa-B p100 subunit;nuclear factor NF-kappa-B p52 subunit;nuclear factor of Kappa light chain gene enhancer in B cells 2;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2;nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100);oncogene Lyt-10;p100;p49/p100;p52;transcription factor NFKB2.
• Product Format: Liquid
• Reference: Qu Z, et al. (2004) J Biol Chem. 279(43): 44563-44572.
  Xiao G, et al. (2001) Mol Cell. 7(2): 401-409.
  Baeuerle P A, et al. (1994) Annu Rev Immunol. 12:141-179.
  Baeuerle P A, et al. (1996) Cell 87:13-20.
  Haskill S, et al. (1991) Cell 65:128
• Description of Target: NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. In concert with RELB, regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer.
• Reconstitution and Storage: -20°C
• Specificity: NF-?B p100/p52 (Ab-865) Antibody detects endogenous levels of NF-?B p100/p52 protein around Serine 865.
• Application Info: WB: 1:500~1:3000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:20000; IP: Various Dilution
• Immunogen: The antiserum was produced against synthesized peptide derived from human NF-kappaB p100/p52.
• Dilution: The optimal working dilution should be determined by the end user.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Uniprot ID: Q00653
• Protein Name: Nuclear factor NF-kappa-B p100 subunit
• Protein Accession #: NP_001070962.1
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Gene Symbol: NFKB2
• Predicted Species Reactivity: Human|Mouse|Rat
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Paraffin|Immunoprecipitation|Western blot
• Image 1: human breast carcinoma tissue
  
  Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using NF-kappaB p100/p52 Antibody. The picture on the right is treated with the synthesized peptide.
• Image 2: ovary cancer cells
  
  Western blot analysis of extracts from ovary cancer cells, using NF-kappaB p100/p52 Antibody. The lane on the right is treated with the synthesized peptide.