| Product Number |
OAAF01349 |
| Product Page |
www.avivasysbio.com/jun-antibody-oaaf01349.html |
| Name |
JUN Antibody (OAAF01349) |
| Molecular Weight |
35 kDa |
| NCBI Gene Id |
3725 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Gene Full Name |
Jun proto-oncogene, AP-1 transcription factor subunit |
| Alias Symbols |
activator protein 1;AP1;AP-1;c-Jun;cJUN;enhancer-binding protein AP1;Jun activation domain binding protein;jun oncogene;p39;proto-oncogene cJun;proto-oncogene c-Jun;transcription factor AP-1;v-jun avian sarcoma virus 17 oncogene homolog;v-jun sarcoma virus 17 oncogene homolog. |
| Product Format |
Liquid |
| Reference |
Boyle W J, et al. (1991) Cell. 64(3): 573-584.
Binetruy B, et al. (1991) Nature. 351: 122-127.
Smeal T, et al. (1991) Nature. 354:494-496.
Derijard B, et al. (1994) Cell. 76:1025-1037.
Kyriakis J M, et al. (1994) Nature. 369: 156-160. |
| Description of Target |
Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306). |
| Reconstitution and Storage |
-20°C |
| Datasheets/Manuals |
Printable datasheet for JUN Antibody (OAAF01349) |
| Specificity |
c-Jun (Ab-243) antibody detects endogenous levels of total c-Jun protein. |
| Application Info |
WB: 1:500~1:3000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:20000; IP: Various Dilution |
| Immunogen |
The antiserum was produced against synthesized peptide derived from human c-Jun. |
| Dilution |
The optimal working dilution should be determined by the end user. |
| Formulation |
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Uniprot ID |
P05412 |
| Protein Name |
Transcription factor AP-1 |
| Protein Accession # |
NP_002219.1 |
| Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Gene Symbol |
JUN |
| Predicted Species Reactivity |
Human|Mouse|Rat |
| Application |
Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Immunoprecipitation|Western blot |
| Image 1 | HeLa cells
 | Western blot analysis of extracts from HeLa cells, using c-Jun Antibody. The lane on the right is treated with the synthesized peptide. |
|
| Image 2 | human brain tissue
 | Immunohistochemistry analysis of paraffin-embedded human brain tissue, using c-Jun Antibody. The picture on the right is treated with the synthesized peptide. |
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