ITGB3 Antibody (OAAF01337)

Data Sheet
 
Product Number OAAF01337
Product Page www.avivasysbio.com/itgb3-antibody-oaaf01337.html
Name ITGB3 Antibody (OAAF01337)
Molecular Weight 87 kDa
NCBI Gene Id 3690
Host Rabbit
Clonality Polyclonal
Gene Full Name integrin subunit beta 3
Alias Symbols antigen CD61;BDPLT16;BDPLT2;BDPLT24;CD61;GP3A;GPIIIa;GT;GT2;integrin beta 3;integrin beta chain, beta 3;integrin beta-3;integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61);platelet membrane glycoprotein IIIa.
Product Format Liquid
Reference Fitzgerald L.A., J. Biol. Chem. 262:3936-3939(1987).
Zimrin A.B., J. Clin. Invest. 81:1470-1475(1988).
Frachet P., Mol. Biol. Rep. 14:27-33(1990).
Description of Target Integrin alpha-V/beta-3 (ITGAV:ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 (ITGA2B:ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. Fibrinogen binding enhances SELP expression in activated platelets (By similarity). ITGAV:ITGB3 binds to fractalkine (CX3CL1) and acts as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415, PubMed:24789099). ITGAV:ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGAV:ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed:18441324). ITGAV:ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling (PubMed:28302677). ITGAV:ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:19578119). ITGAV:ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed:28873464). ITGAV:ITGB3 binds to IL1B and this binding is essential for IL1B signaling (PubMed:29030430). ITGAV:ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGAV:ITGB3 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887).
Reconstitution and Storage -20°C
Datasheets/Manuals Printable datasheet for ITGB3 Antibody (OAAF01337)
Specificity Integrin beta3 Antibody detects endogenous levels of total Integrin beta3 protein.
Application Info WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:20000
The optimal working dilution should be determined by the end user.
Immunogen The antiserum was produced against synthesized peptide derived from human Integrin beta3.
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Uniprot ID P05106
Protein Name Integrin beta-3
Protein Accession # NP_000203.2
Purification The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
Gene Symbol ITGB3
Predicted Species Reactivity Human|Mouse
Application Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
Image 1
human breast carcinoma tissue
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Integrin beta3 Antibody. The picture on the right is treated with the synthesized peptide.
Image 2
HepG2 cells
Western blot analysis of extracts from HepG2 cells, using Integrin beta3 Antibody. The lane on the right is treated with the synthesized peptide.
 

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