| Product Number |
OAAF01183 |
| Product Page |
www.avivasysbio.com/npm1-antibody-oaaf01183.html |
| Name |
NPM1 Antibody (OAAF01183) |
| Molecular Weight |
32 kDa |
| Isotype |
IgG |
| NCBI Gene Id |
4869 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Gene Full Name |
nucleophosmin 1 |
| Alias Symbols |
B23;NPM;Nucleolar phosphoprotein B23;nucleolar protein NO38;nucleophosmin;nucleophosmin (nucleolar phosphoprotein B23, numatrin);nucleophosmin/nucleoplasmin family, member 1;Numatrin;testicular tissue protein Li 128. |
| Product Format |
Liquid |
| Reference |
Chan P.-K., Nucleic Acids Res. 25:1225-1232(1997).
Falini B., N. Engl. J. Med. 352:254-266(2005).
Bolli N., cet Oncol. 7:350-352(2006). |
| Description of Target |
Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486). In complex with MYC enhances the transcription of MYC target genes (PubMed:25956029). |
| Reconstitution and Storage |
-20°C |
| Datasheets/Manuals |
Printable datasheet for NPM1 Antibody (OAAF01183) |
| Specificity |
NPM antibody detects endogenous levels of total NPM protein. |
| Application Info |
WB: 1:500~1:1000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:1000 |
| Immunogen |
The antiserum was produced against synthesized peptide derived from human NPM. |
| Dilution |
The optimal working dilution should be determined by the end user. |
| Formulation |
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Uniprot ID |
P06748 |
| Protein Name |
Nucleophosmin |
| Protein Accession # |
NP_001032827.1 |
| Purification |
The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen. |
| Gene Symbol |
NPM1 |
| Predicted Species Reactivity |
Human|Mouse|Rat |
| Application |
Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot |
| Image 1 | HeLa/NIH-3T3 cells
 | Western blot analysis of extracts from HeLa/NIH-3T3 cells, using NPM Antibody. The lane on the right is treated with the synthesized peptide. |
|
| Image 2 | human tonsil tissue
 | Immunohistochemistry analysis of paraffin-embedded human tonsil tissue, using NPM Antibody. The picture on the right is treated with the synthesized peptide. |
|
| Image 3 | HeLa cells
 | Immunofluorescence analysis of HeLa cells, using NPM Antibody. The picture on the right is treated with the synthesized peptide. |
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