IRF3 Antibody (OAAF00779)

• Product Number: OAAF00779
• Name: IRF3 Antibody (OAAF00779)
• Molecular Weight: 47 kDa
• NCBI Gene Id: 3661
• Host: Rabbit
• Clonality: Polyclonal
• Gene Full Name: interferon regulatory factor 3
• Alias Symbols: IIAE7;interferon regulatory factor 3.
• Product Format: Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Reference: Wen-Shuz Yeow, J. Virol., Mar 2001; 75: 3021 - 3027.
  Subhra K. Biswas, Blood, Mar 2006; 107: 2112 - 2122.
  Benjamin R. tenOever, J. Virol., Apr 2002; 76: 3659 - 3669
• Description of Target: Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.
• Reconstitution and Storage: -20°C
• Specificity: IRF-3 (Ab-385) antibody detects endogenous levels of total IRF-3 protein
• Application Info: WB 1:500~1:1000
  IHC 1:50~1:100
  IF 1:100~1:500
  ELISA 1:5000
• Immunogen: The antiserum was produced against synthesized peptide derived from human IRF3.
• Uniprot ID: Q14653
• Protein Name: Interferon regulatory factor 3
• Publications:

  Dong, X.-Y. et al. Classical swine fever virus triggers RIG-I and MDA5-dependent signaling pathway to IRF-3 and NF-κB activation to promote secretion of interferon and inflammatory cytokines in porcine alveolar macrophages. Virol. J. 10, 286 (2013). 24034559

• Protein Accession #: NP_001184053.1
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Gene Symbol: IRF3
• Predicted Species Reactivity: Human|Mouse|Rat
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Image 1: HeLa cells
  
  Immunofluorescence analysis of HeLa cells, using IRF3 Antibody. The picture on the right is treated with the synthesized peptide.
• Image 2: human lung carcinoma tissue
  
  Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using IRF3 Antibody. The picture on the right is treated with the synthesized peptide.
• Image 3: K562 cells
  
  Western blot analysis of extracts from K562 cells, using IRF3 Antibody. The lane on the right is treated with the synthesized peptide.