ABL1 Antibody (OAAF00709)

Data Sheet
 
Product Number OAAF00709
Product Page www.avivasysbio.com/abl1-antibody-oaaf00709.html
Name ABL1 Antibody (OAAF00709)
Molecular Weight 122 kDa
NCBI Gene Id 25|27
Host Rabbit
Clonality Polyclonal
Gene Full Name ABL proto-oncogene 1, non-receptor tyrosine kinase|ABL proto-oncogene 2, non-receptor tyrosine kinase
Alias Symbols Abelson murine leukemia viral oncogene homolog 1;Abelson murine leukemia viral oncogene homolog 2;Abelson tyrosine-protein kinase 1;Abelson tyrosine-protein kinase 2;abelson-related gene protein;ABL;ABL protooncogene 1 nonreceptor tyrosine kinase;ABLL;ARG;bcr/abl;bcr/c-abl oncogene protein;BCR-ABL;c-ABL;c-abl oncogene 1, receptor tyrosine kinase;c-abl oncogene 2, non-receptor tyrosine kinase;c-ABL1;CHDSKM;JTK7;p150;proto-oncogene c-Abl;proto-oncogene tyrosine-protein kinase ABL1;truncated ABL protooncogene 1 nonreceptor tyrosine kinase;tyrosine-protein kinase ABL1;tyrosine-protein kinase ABL2;tyrosine-protein kinase ARG;v-abl;v-abl Abelson murine leukemia viral oncogene homolog 1;v-abl Abelson murine leukemia viral oncogene homolog 2.
Product Format Liquid
Reference Fainstein E., Oncogene 4:1477-1481(1989).
Shtivelman E., Cell 47:277-284(1986).
Chissoe S.L., Genomics 27:67-82(1995).
Description of Target Non-receptor tyrosine-protein kinase that plays a role in many key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion, receptor endocytosis, autophagy, DNA damage response and apoptosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like WASF3 (involved in branch formation); ANXA1 (involved in membrane anchoring); DBN1, DBNL, CTTN, RAPH1 and ENAH (involved in signaling); or MAPT and PXN (microtubule-binding proteins). Phosphorylation of WASF3 is critical for the stimulation of lamellipodia formation and cell migration. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as BCAR1, CRK, CRKL, DOK1, EFS or NEDD9. Phosphorylates multiple receptor tyrosine kinases and more particularly promotes endocytosis of EGFR, facilitates the formation of neuromuscular synapses through MUSK, inhibits PDGFRB-mediated chemotaxis and modulates the endocytosis of activated B-cell receptor complexes. Other substrates which are involved in endocytosis regulation are the caveolin (CAV1) and RIN1. Moreover, ABL1 regulates the CBL family of ubiquitin ligases that drive receptor down-regulation and actin remodeling. Phosphorylation of CBL leads to increased EGFR stability. Involved in late-stage autophagy by regulating positively the trafficking and function of lysosomal components. ABL1 targets to mitochondria in response to oxidative stress and thereby mediates mitochondrial dysfunction and cell death. In response to oxidative stress, phosphorylates serine/threonine kinase PRKD2 at 'Tyr-717' (PubMed:28428613). ABL1 is also translocated in the nucleus where it has DNA-binding activity and is involved in DNA-damage response and apoptosis. Many substrates are known mediators of DNA repair: DDB1, DDB2, ERCC3, ERCC6, RAD9A, RAD51, RAD52 or WRN. Activates the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates TP73, a primary regulator for this type of damage-induced apoptosis. Phosphorylates the caspase CASP9 on 'Tyr-153' and regulates its processing in the apoptotic response to DNA damage. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks. ABL1 acts also as a regulator of multiple pathological signaling cascades during infection. Several known tyrosine-phosphorylated microbial proteins have been identified as ABL1 substrates. This is the case of A36R of Vaccinia virus, Tir (translocated intimin receptor) of pathogenic E.coli and possibly Citrobacter, CagA (cytotoxin-associated gene A) of H.pylori, or AnkA (ankyrin repeat-containing protein A) of A.phagocytophilum. Pathogens can highjack ABL1 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1. Regulates T-cell differentiation in a TBX21-dependent manner. Phosphorylates TBX21 on tyrosine residues leading to an enhancement of its transcriptional activator activity (By similarity).|Non-receptor tyrosine-protein kinase that plays an ABL1-overlapping role in key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion and receptor endocytosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like MYH10 (involved in movement); CTTN (involved in signaling); or TUBA1 and TUBB (microtubule subunits). Binds directly F-actin and regulates actin cytoskeletal structure through its F-actin-bundling activity. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as CRK, CRKL, DOK1 or ARHGAP35. Adhesion-dependent phosphorylation of ARHGAP35 promotes its association with RASA1, resulting in recruitment of ARHGAP35 to the cell periphery where it inhibits RHO. Phosphorylates multiple receptor tyrosine kinases like PDGFRB and other substrates which are involved in endocytosis regulation such as RIN1. In brain, may regulate neurotransmission by phosphorylating proteins at the synapse. ABL2 acts also as a regulator of multiple pathological signaling cascades during infection. Pathogens can highjack ABL2 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1.
Reconstitution and Storage -20°C
Datasheets/Manuals Printable datasheet for ABL1 Antibody (OAAF00709)
Specificity Abl antibody detects endogenous levels of total Abl protein.
Application Info IHC: 1:50~1:100; ELISA: 1:10000
Immunogen The antiserum was produced against synthesized peptide derived from human Abl.
Dilution The optimal working dilution should be determined by the end user.
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Uniprot ID P00519|P42684
Protein Name Tyrosine-protein kinase ABL1|Tyrosine-protein kinase ABL2
Protein Accession # NP_005148.2
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Gene Symbol ABL1|ABL2
Predicted Species Reactivity Human|Mouse
Application Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin
Image 1
human lung carcinoma tissue
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Abl Antibody. The picture on the right is treated with the synthesized peptide.
 

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