| Product Number |
OAAF00681 |
| Product Page |
www.avivasysbio.com/ccnb1-antibody-oaaf00681.html |
| Name |
CCNB1 Antibody (OAAF00681) |
| Molecular Weight |
48 kDa |
| NCBI Gene Id |
891 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Gene Full Name |
cyclin B1 |
| Alias Symbols |
CCNB;G2/mitotic-specific cyclin-B1. |
| Product Format |
Liquid |
| Reference |
Jing Yang, J. Biol. Chem., Jan 2001; 276: 3604 - 3609.
Susan Walsh, Mol. Cancer Res., Feb 2003; 1: 280.
Florian Bassermann, J. Biol. Chem., Mar 2007; 10.1074.
Jianke Li, PNAS, Jan 1997; 94: 502. |
| Description of Target |
Essential for the control of the cell cycle at the G2/M (mitosis) transition. |
| Reconstitution and Storage |
-20°C |
| Datasheets/Manuals |
Printable datasheet for CCNB1 Antibody (OAAF00681) |
| Specificity |
Cyclin B1 (Ab-126) antibody detects endogenous levels of total Cyclin B1 protein. |
| Application Info |
WB: 1:500~1:3000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:20000 |
| Immunogen |
The antiserum was produced against synthesized peptide derived from human Cyclin B1. |
| Dilution |
The optimal working dilution should be determined by the end user. |
| Formulation |
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Uniprot ID |
P14635 |
| Protein Name |
G2/mitotic-specific cyclin-B1 |
| Protein Accession # |
NP_114172.1 |
| Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Gene Symbol |
CCNB1 |
| Predicted Species Reactivity |
Human|Mouse|Rat |
| Application |
Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot |
| Image 1 | HeLa cells
 | Immunofluorescence analysis of HeLa cells, using Cyclin B1 Antibody. The picture on the right is treated with the synthesized peptide. |
|
| Image 2 | human breast carcinoma tissue
 | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Cyclin B1 Antibody. The picture on the right is treated with the synthesized peptide. |
|
| Image 3 | K562 cells
 | Western blot analysis of extracts from K562 cells, treated with serum 10% 15', using Cyclin B1 Antibody. The lane on the right is treated with the synthesized peptide. |
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