Ephrin B1/B2/B3 Antibody (Phospho-Tyr324) (OAAF00520)

• Product Number: OAAF00520
• Name: Ephrin B1/B2/B3 Antibody (Phospho-Tyr324) (OAAF00520)
• Molecular Weight: 38 kDa
• Isotype: IgG
• NCBI Gene Id: 1947|1948|1949
• Host: Rabbit
• Clonality: Polyclonal
• Gene Full Name: ephrin B1|ephrin B2|ephrin B3
• Description:
  View product citations for Ephrin B1/B2/B3 Antibody (Phospho-Tyr324) (OAAF00520) on CiteAb
• Alias Symbols: CFND;CFNS;EFB1;EFL3;EFL-3;EFL6;ELK ligand;Elk-L;EPH-related receptor transmembrane ligand ELK-L3;eph-related receptor tyrosine kinase ligand 2;eph-related receptor tyrosine kinase ligand 5;eph-related receptor tyrosine kinase ligand 8;ephrin-B1;ephrin-B2;ephrin-B3;EPLG2;EPLG5;EPLG8;HTK ligand;Htk-L;HTKL;LERK2;LERK5;LERK8;ligand of eph-related kinase 5.
• Product Format: Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Reference: Beckmann M.P., EMBO J. 13:3757-3762(1994).
  Davis S., Science 266:816-819(1994).
  Fletcher F.A., Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
• Description of Target: Binds to the receptor tyrosine kinases EPHB1 and EPHA1. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons (By similarity).|Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.|Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. May play a pivotal role in forebrain function. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons (By similarity).
• Reconstitution and Storage: -20°C
• Specificity: Ephrin B1/B2/B3 (Phospho-Tyr324) antibody detects endogenous levels of Ephrin B1/B2/B3 only when phosphorylated at tyrosine 324.
• Additional Information: Target Modification: Phospho
  Modification Sites: H:Y324/311/318 M:Y323/314/318 R:Y323/-/318
  
• Application Info: WB: 1:500~1:1000
  IHC: 1:50~1:100
  ELISA: 1:20000
• Immunogen: The antiserum was produced against synthesized peptide derived from human Ephrin B1/B2/B3 around the phosphorylation site of Tyr324.
• Dilution: The optimal working dilution should be determined by the end user.
• Uniprot ID: P52799|P98172|Q15768
• Protein Name: Ephrin-B1|Ephrin-B2|Ephrin-B3
• Publications:

  Activating Mutations in PIK3CA Lead to Widespread Modulation of the Tyrosine Phosphoproteome. J. Proteome Res. 14, 3882-91 (2015). 26267517

• Protein Accession #: NP_001397.1
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Gene Symbol: EFNB1|EFNB2|EFNB3
• Predicted Species Reactivity: Human|Mouse|Rat
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Image 1: Human Brain
  
  Immunohistochemistry analysis of paraffin-embedded human brain, using Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody. The picture on the right is blocked with the synthesized peptide.
• Image 2: K562 cells
  
  Western blot analysis of extracts from K562 cells treated with serum 20% 15', using Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody. The lane on the right is blocked with the synthesized peptide.