DDR1 Antibody (Phospho-Tyr513) (OAAF00504)

• Product Number: OAAF00504
• Name: DDR1 Antibody (Phospho-Tyr513) (OAAF00504)
• Molecular Weight: 101 kDa
• NCBI Gene Id: 780
• Host: Rabbit
• Clonality: Polyclonal
• Gene Full Name: discoidin domain receptor tyrosine kinase 1
• Alias Symbols: CAK;CD167;CD167 antigen-like family member A;cell adhesion kinase;DDR;Discoidin receptor tyrosine kinase;EDDR1;epithelial discoidin domain-containing receptor 1;HGK2;mammary carcinoma kinase 10;MCK10;NEP;neuroepithelial tyrosine kinase;neurotrophic tyrosine kinase, receptor, type 4;NTRK4;Protein-tyrosine kinase 3A;protein-tyrosine kinase RTK-6;PTK3;PTK3A;PTK3A protein tyrosine kinase 3A;RTK6;TRK E;TRKE;tyrosine kinase DDR;tyrosine-protein kinase CAK.
• Product Format: Liquid
• Reference: di Marco E., J. Biol. Chem. 268:24290-24295(1993).
  Johnson J.D., Proc. Natl. Acad. Sci. U.S.A. 90:5677-5681(1993).
  Laval S., Cell Growth Differ. 5:1173-1183(1994).
• Description of Target: Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing. Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing (By similarity). Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Also plays a role in tumor cell invasion. Phosphorylates PTPN11.
• Reconstitution and Storage: -20°C
• Specificity: DDR1 (Phospho-Tyr513) antibody detects endogenous levels of DDR1 only when phosphorylated at tyrosine 513.
• Application Info: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:10000
• Immunogen: The antiserum was produced against synthesized peptide derived from human DDR1 around the phosphorylation site of Tyr513.
• Dilution: The optimal working dilution should be determined by the end user.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Uniprot ID: Q08345
• Protein Name: Epithelial discoidin domain-containing receptor 1
• Protein Accession #: NP_001189450.1
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Gene Symbol: DDR1
• Predicted Species Reactivity: Human|Mouse|Rat
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Image 1: human brain
  
  Immunohistochemistry analysis of paraffin-embedded human brain, using DDR1 (Phospho-Tyr513) Antibody. The picture on the right is treated with the synthesized peptide.
• Image 2: HepG2 cells
  
  Western blot analysis of extracts from HepG2 cells treated with Na3VO4 0.3mM 40', using DDR1 (Phospho-Tyr513) Antibody. The lane on the right is treated with the synthesized peptide.