RB1 Antibody (Phospho-Ser608) (OAAF00421)

• Product Number: OAAF00421
• Name: RB1 Antibody (Phospho-Ser608) (OAAF00421)
• Molecular Weight: 106 kDa
• Isotype: IgG
• NCBI Gene Id: 5925
• Host: Rabbit
• Clonality: Polyclonal
• Gene Full Name: RB transcriptional corepressor 1
• Alias Symbols: exon 17 tumor GOS561 substitution mutation causes premature stop;GOS563 exon 17 substitution mutation causes premature stop;OSRC;p105-Rb;p110-RB1;pp110;PPP1R130;pRb;prepro-retinoblastoma-associated protein;protein phosphatase 1, regulatory subunit 130;RB;retinoblastoma 1;retinoblastoma suspectibility protein;retinoblastoma-associated protein.
• Product Format: Liquid. Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Reference: Lee W.-H., Nature 329:642-645(1987).
  Lee W.-H., Science 235:1394-1399(1987).
  Friend S.H., Proc. Natl. Acad. Sci. U.S.A. 84:9059-9063(1987).
• Description of Target: Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
• Reconstitution and Storage: -20°C
• Specificity: Retinoblastoma (Phospho-Ser608) antibody detects endogenous levels of Retinoblastoma only when phosphorylated at serine 608.
• Additional Information: Modification Sites: H:S608 M:S601 R:S579
• Application Info: WB: 1:500~1:1000
  IHC: 1:50~1:100
  
  ELISA: 1:40000
• Immunogen: The antiserum was produced against synthesized peptide derived from human Retinoblastoma around the phosphorylation site of Ser608.
• Dilution: The optimal working dilution should be determined by the end user.
• Uniprot ID: P06400
• Protein Name: Retinoblastoma-associated protein
• Protein Accession #: NP_000312.2
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Gene Symbol: RB1
• Predicted Species Reactivity: Human|Mouse|Rat
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Image 1: Jurkat cells
  
  Western blot analysis of extracts from Jurkat cells treated with PMA 125ng/ml 30', using Retinoblastoma (Phospho-Ser608) Antibody. The lane on the right is blocked with the synthesized peptide.
• Image 2: human brain
  
  Immunohistochemistry analysis of paraffin-embedded human brain, using Retinoblastoma (Phospho-Ser608) Antibody. The picture on the right is blocked with the synthesized peptide.