CAMK2D Antibody (Phospho-Thr286) (OAAF00291)

Data Sheet
 
Product Number OAAF00291
Product Page www.avivasysbio.com/camk2d-antibody-phospho-thr286-oaaf00291.html
Name CAMK2D Antibody (Phospho-Thr286) (OAAF00291)
Molecular Weight 54 kDa
Isotype IgG
NCBI Gene Id 815|817
Host Rabbit
Clonality Polyclonal
Gene Full Name calcium/calmodulin dependent protein kinase II alpha|calcium/calmodulin dependent protein kinase II delta
Alias Symbols calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha;calcium/calmodulin-dependent protein kinase (CaM kinase) II delta;calcium/calmodulin-dependent protein kinase II alpha-B subunit;calcium/calmodulin-dependent protein kinase type II alpha chain;calcium/calmodulin-dependent protein kinase type II delta chain;calcium/calmodulin-dependent protein kinase type II subunit alpha;calcium/calmodulin-dependent protein kinase type II subunit delta;CaM kinase II alpha subunit;CaM kinase II delta subunit;caM kinase II subunit alpha;CAMKA;CAMKD;CaMK-II alpha subunit;CaMK-II delta subunit;caMK-II subunit alpha;CaMKIIalpha;CaMKIINalpha;CaM-kinase II alpha chain;CaM-kinase II delta chain;MRD53;MRT63.
Product Format Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Reference Li G.Y., Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
Nagase T., DNA Res. 6:63-70(1999).
Krapivinsky G., Neuron 43:563-574(2004).
Description of Target Calcium/calmodulin-dependent protein kinase involved in the regulation of Ca(2+) homeostatis and excitation-contraction coupling (ECC) in heart by targeting ion channels, transporters and accessory proteins involved in Ca(2+) influx into the myocyte, Ca(2+) release from the sarcoplasmic reticulum (SR), SR Ca(2+) uptake and Na(+) and K(+) channel transport. Targets also transcription factors and signaling molecules to regulate heart function. In its activated form, is involved in the pathogenesis of dilated cardiomyopathy and heart failure. Contributes to cardiac decompensation and heart failure by regulating SR Ca(2+) release via direct phosphorylation of RYR2 Ca(2+) channel on 'Ser-2808'. In the nucleus, phosphorylates the MEF2 repressor HDAC4, promoting its nuclear export and binding to 14-3-3 protein, and expression of MEF2 and genes involved in the hypertrophic program. Is essential for left ventricular remodeling responses to myocardial infarction. In pathological myocardial remodeling acts downstream of the beta adrenergic receptor signaling cascade to regulate key proteins involved in ECC. Regulates Ca(2+) influx to myocytes by binding and phosphorylating the L-type Ca(2+) channel subunit beta-2 CACNB2. In addition to Ca(2+) channels, can target and regulate the cardiac sarcolemmal Na(+) channel Nav1.5/SCN5A and the K+ channel Kv4.3/KCND3, which contribute to arrhythmogenesis in heart failure. Phosphorylates phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2, contributing to the enhancement of SR Ca(2+) uptake that may be important in frequency-dependent acceleration of relaxation (FDAR) and maintenance of contractile function during acidosis. May participate in the modulation of skeletal muscle function in response to exercise, by regulating SR Ca(2+) transport through phosphorylation of PLN/PLB and triadin, a ryanodine receptor-coupling factor.|CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity (By similarity). Phosphorylates transcription factor FOXO3 on 'Ser-298' (PubMed:23805378). Activates FOXO3 transcriptional activity (By similarity).
Reconstitution and Storage -20°C
Datasheets/Manuals Printable datasheet for CAMK2D Antibody (Phospho-Thr286) (OAAF00291)
Specificity CaMK2 (Phospho-Thr286) antibody detects endogenous levels of CaMK2 only when phosphorylated at threonine 286.
Additional Information Modification Sites: H:T286/287 M:T286/287 R:T286/287
Application Info WB 1:500~1000
IHC 1:50~100
IF 1:100~500
ELISA 1:10000
Immunogen The antiserum was produced against synthesized peptide derived from human CaMK2 around the phosphorylation site of Thr286.
Dilution The optimal working dilution should be determined by the end user.
Uniprot ID Q13557|Q9UQM7
Protein Name Calcium/calmodulin-dependent protein kinase type II subunit alpha|Calcium/calmodulin-dependent protein kinase type II subunit delta
Protein Accession # NP_001212.2
Purification The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Gene Symbol CAMK2A|CAMK2D
Predicted Species Reactivity Human|Mouse|Rat
Application Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
Image 1
K562 cells
Western blot analysis of extracts from K562 cells, using CaMK2 (Phospho-Thr286) Antibody. The lane on the right is treated with the synthesized peptide.
Image 2
human brain
Immunohistochemistry analysis of paraffin-embedded human brain, using CaMK2 (Phospho-Thr286) Antibody. The picture on the right is treated with the synthesized peptide.
Image 3
COS7 cells
Immunofluorescence analysis of COS7 cells, using CaMK2 (Phospho-Thr286) Antibody. The picture on the right is treated with the synthesized peptide.
 

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