ACO1 Antibody (Phospho-Ser711) (OAAF00239)

• Product Number: OAAF00239
• Name: ACO1 Antibody (Phospho-Ser711) (OAAF00239)
• Molecular Weight: 98 kDa
• NCBI Gene Id: 48
• Host: Rabbit
• Clonality: Polyclonal
• Gene Full Name: aconitase 1
• Alias Symbols: aconitase 1, soluble;aconitate hydratase, cytoplasmic;ACONS;citrate hydro-lyase;cytoplasmic aconitase;cytoplasmic aconitate hydratase;cytosplasmic aconitase;epididymis luminal protein 60;epididymis secretory sperm binding protein;ferritin repressor protein;HEL60;IREB1;IREBP;IREBP1;iron regulatory protein 1;iron-responsive element-binding protein 1;IRP1;soluble aconitase.
• Product Format: Liquid
• Reference: Hirling H., Nucleic Acids Res. 20:33-39(1992).
  Rouault T.A., Proc. Natl. Acad. Sci. U.S.A. 87:7958-7962(1990).
  Hentze M.W., Nucleic Acids Res. 19:1739-1740(1991)
• Description of Target: Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding.
• Reconstitution and Storage: -20°C
• Specificity: IREB1 (Phospho-Ser711) antibody detects endogenous levels of IREB1 only when phosphorylated at serine 711.
• Additional Information: Modification Sites: Human:S711; Mouse:S711; Rat:S711
• Application Info: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:10000
• Immunogen: The antiserum was produced against synthesized peptide derived from human IREB1 around the phosphorylation site of Ser711.
• Dilution: The optimal working dilution should be determined by the end user.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Uniprot ID: P21399
• Protein Name: Cytoplasmic aconitate hydratase
• Protein Accession #: NP_002188.1
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Gene Symbol: ACO1
• Predicted Species Reactivity: Human|Monkey|Mouse|Rat
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Image 1: human thyroid gland
  
  Immunohistochemistry analysis of paraffin-embedded human thyroid gland, using IREB1 (Phospho-Ser711) Antibody. The picture on the right is treated with the synthesized peptide.
• Image 2: 293 cells
  
  Western blot analysis of extracts from 293 cells treated with insulin 0.01U/ml 30', using IREB1 (Phospho-Ser711) Antibody. The lane on the right is treated with the synthesized peptide.