NTRK2 Antibody (Phospho-Tyr515) (OAAF00024)

• Product Number: OAAF00024
• Name: NTRK2 Antibody (Phospho-Tyr515) (OAAF00024)
• Molecular Weight: 91 kDa
• Isotype: IgG
• NCBI Gene Id: 4915
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Gene Full Name: neurotrophic receptor tyrosine kinase 2
• Alias Symbols: BDNF/NT-3 growth factors receptor;BDNF-tropomyosine receptor kinase B;DEE58;EIEE58;GP145-TrkB;neurotrophic tyrosine kinase receptor type 2;OBHD;trk-B;TRKB;TrkB tyrosine kinase;tropomyosin-related kinase B;tyrosine kinase receptor B.
• Product Format: Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Reference: Tina Garofalo, J. Biol. Chem., Dec 1998; 273: 35153.
  VI Shifrin and BG Neel, J. Biol. Chem., Dec 1993; 268: 25376 - 25384.
  Jan Grimm, J. Cell Biol., Jul 2001; 154: 345.
  Jose L. Tomsig and Terry T. Turner, J. Androl, May 2006; 27: 348 - 357
• Description of Target: Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. May also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia.
• Reconstitution and Storage: -20°C
• Specificity: Trk B (Phospho-Tyr515) Antibody detects endogenous levels of Trk B only when phosphorylated at Tyr515.
• Additional Information: Modification Sites: H: Y516, M: Y515, R: Y515
• Application Info: WB: 1:500~1:1000
  IHC: 1:50~1:100
  ELISA: 1:5000
  Optimal dilutions should be determined by the end user.
• Immunogen: The antiserum was produced against synthesized peptide derived from human Trk B around the phosphorylation site of Tyr515.
• Dilution: The optimal working dilution should be determined by the end user.
• Uniprot ID: Q16620
• Protein Name: BDNF/NT-3 growth factors receptor
• Protein Accession #: NP_001007098.1
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Target Post-Translational Modification: Phospho
• Gene Symbol: NTRK2
• Predicted Species Reactivity: Human|Mouse|Rat
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Image 1: human brain
  
  Immunohistochemistry analysis of paraffin-embedded human brain, using Trk B (Phospho-Tyr515) Antibody. The picture on the right is blocked with the synthesized peptide.
• Image 2: NIH-3T3 cells
  
  Western blot analysis of extracts from NIH-3T3 cells, using Trk B (Phospho-Tyr515) Antibody. The lane on the right is blocked with the synthesized peptide.