KLF4 Antibody (OAAD00376)

• Product Number: OAAD00376
• Name: KLF4 Antibody (OAAD00376)
• Molecular Weight: 54kDa
• Clone: 1E6
• Isotype: IgG1
• NCBI Gene Id: 9314
• Host: Mouse
• Clonality: Monoclonal
• Alias Symbols: EZF, GKLF
• Reference: 1. Mol Cell Proteomics. 2008 Mar;7(3):499-508.
  2. J Cancer Res Clin Oncol. 2008 Aug;134(8):891-8.
• Description of Target: Kruppel-like factor 4 (gut), endothelial Kruppel-like zinc finger protein. May act as a transcriptional activator. Binds the CACCC core sequence. May be involved in the differentiation of epithelial cells and may also function in the development of the skeleton and kidney. KLF4 is highly expressed in the epithelial cells of the skin and the gastrointestinal tract.
• Reconstitution and Storage: Store at 4C, for long term storage, store at -20C.
• Application Info: Western Bloting: 1/500 - 1/2000.
  Immunohistochemistry: 1/200 - 1/1000.
  Immunofluorescence: 1/200 - 1/1000.
  ELISA: Propose dilution 1/10000.
  Not yet tested in other applications.
  Determining optimal working dilutions by titration test.
• Immunogen: Purified recombinant fragment of human KLF4 expressed in E. Coli.
• Formulation: Ascitic fluid containing 0.03% sodium azide.
• Uniprot ID: O43474
• Publications:

  Luz-Madrigal, A. et al. Reprogramming of the chick retinal pigmented epithelium after retinal injury. BMC Biol. 12, 28 (2014). 24742279

• Gene Symbol: KLF4
• Predicted Species Reactivity: Human
• Application: WB, IHC, IF
• Image 1: colon cancer
  
  Immunohistochemical analysis of paraffin-embedded colon cancer tissues (left) and lung cancer tissues (right) using KLF4 mouse mAb with DAB staining
• Image 2: ECA109
  
  Immunofluorescence analysis of ECA109 cells using KLF4 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
• Image 3: Transfected HEK293
  
  western blot analysis using KLF4 mAb against HEK293 (1) and KLF4-hIgGFc transfected HEK293 (2) cell lysate.