| Product Number |
OAAD00231 |
| Product Page |
www.avivasysbio.com/gapdh-antibody-oaad00231.html |
| Name |
GAPDH Antibody (OAAD00231) |
| Molecular Weight |
37 kDa |
| Clone |
1A10 |
| Isotype |
IgG1 |
| NCBI Gene Id |
2597 |
| Host |
Mouse |
| Clonality |
Monoclonal |
| Concentration |
0.5 mg/ml |
| Description |
|
| Alias Symbols |
G3PD, GAPD, HEL-S-162eP |
| Product Format |
Liquid containing 0.03% sodium azide. |
| Reference |
1. Allen R.W. J. Biol. Chem. 1987.262:649-653.
2. Sumner CJ. Ann Neurol 2003.54:6 47-54. |
| Description of Target |
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. It catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age- related neuronal diseases, such as Alzheimer's and Huntington's disease. |
| Reconstitution and Storage |
Store at 4°C, for long term storage, store at -20°C. |
| Datasheets/Manuals |
Printable datasheet for GAPDH Antibody (OAAD00231) - OAAD00231 |
| Application Info |
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
IF: 1/200 - 1/1000
ELISA: Propose dilution 1/10000 |
| Immunogen |
Purified recombinant fragment of human GAPDH expressed in E. coli. |
| Uniprot ID |
P04406 |
| Protein Name |
Glyceraldehyde-3-phosphate dehydrogenase |
| Publications |
Expression of Kruppel-like factor 8 and Ki67 in lung adenocarcinoma and prognosis. Exp Ther Med. 14, 1351-1356 (2017). 28810596
Kam, A. et al. Gallic acid protects against endothelial injury by restoring the depletion of DNA methyltransferase 1 and inhibiting proteasome activities. Int. J. Cardiol. 171, 231-42 (2014). 24388544 |
| Gene Symbol |
GAPDH |
| Predicted Species Reactivity |
Human |
| Application |
WB, IHC, IF |
| Image 1 | Hela, A549, A431, MCF-7, K562, Jurkat, HL60, SKN-SH, SKBR-3
 | Western blot analysis using GAPDH mouse mAb against Hela (1), A549 (2), A431 (3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate. |
| | Image 2 | Human breast carcinoma, kidney carcinoma
 | Immunohistochemical analysis of paraffin-embedded human breast carcinoma (left) and kidney carcinoma (right), showing cytoplasmic localization using GAPDH mouse mAb with DAB staining. |
| | Image 3 | methanol-fixed HepG2, Hela
 | Confocal immunofluorescence analysis of methanol-fixed HepG2 (left) and Hela (right) cells using GAPDH mouse mAb (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye. |
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