Product Number |
OAAD00224 |
Product Page |
www.avivasysbio.com/actb-antibody-oaad00224.html |
Name |
ACTB Antibody (OAAD00224) |
Clone |
AC-15 |
Isotype |
IgG1 |
NCBI Gene Id |
60 |
Host |
Mouse |
Clonality |
Monoclonal |
Gene Full Name |
Actin, cytoplasmic 1 |
Alias Symbols |
BRWS1, PS1TP5BP1 |
Product Format |
Lyophilized from 1.2% sodium acetate, with 2 mg/ml BSA and 0.01 mg sodium azide |
Reference |
1. Ng, S.-Y.; Gunning, P.; Eddy, R.; Ponte, P.; Leavitt, J.; Kedes, L.; Shows, T. : Chromosome 7 assignment of the human beta-actin functional gene (ACTB) and the chromosomal dispersion of pseudogenes. (Abstract) Cytogenet. Cell Genet. 40: 712 only, 1985. 2. Procaccio, V.; Salazar, G.; Ono, S.; Styers, M. L.; Gearing, M.; Davila, A.; Jimenez, R.; Juncos, J.; Gutekunst, C.-A.; Meroni, G.; Fontanella, B.; Sontag, E.; Sontag, J. M.; Faundez, V.; Wainer, B. H. : A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia. Am. J. Hum. Genet. 78: 947-960, 2006. 3. Ng, S.-Y.; Gunning, P.; Eddy, R.; Ponte, P.; Leavitt, J.; Shows, T.; Kedes, L. : Evolution of the functional human beta-actin gene and its multi-pseudogene family: conservation of the noncoding regions and chromosomal dispersion of pseudogenes. Molec. Cell. Biol. 5: 2720-2732, 1985. |
Description of Target |
The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al. (1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia. |
Reconstitution and Storage |
Reconstitute with 1.2% sodium acetate or neutral PBS. If 1 mL of PBS is used, the antibody concentration will be 100 ug/mL. Store at -20°C for one year. After reconstitution, at 4°C for three months. It can also be aliquotted and stored frozen at -20°C for a longer time. |
Datasheets/Manuals |
Printable datasheet for anti-ACTB (OAAD00224) antibody |
Specificity |
Human, mouse, rat. No cross reactivity with other proteins. |
Application Info |
WB: 0.25 - 0.5 ug/mL with the appropriate system to detect beta-actin in cells and tissues IHC-P: 0.5 - 1 ug/mL to detect beta-actin in formalin fixed and paraffin embedded tissues. |
Immunogen |
Slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH. |
Uniprot ID |
P60709 |
Protein Name |
Actin, cytoplasmic 1 |
Publications |
Selective cytoprotective effect of histamine on doxorubicin-induced hepatic and cardiac toxicity in animal models. Cell Death Discov. 1, 15059 (2015). 27551485 |
Protein Accession # |
NP_001092.1 |
Purification |
Purified by the goat anti-mouse IgG Affinity chromatography. |
Nucleotide Accession # |
NM_001101.3 |
Gene Symbol |
ACTB |
Predicted Species Reactivity |
Human, Mouse, Rat |
Application |
WB, IHC, IP |
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