ACTB Antibody (OAAD00224)

Data Sheet
 
Product Number OAAD00224
Product Page www.avivasysbio.com/actb-antibody-oaad00224.html
Name ACTB Antibody (OAAD00224)
Clone AC-15
Isotype IgG1
NCBI Gene Id 60
Host Mouse
Clonality Monoclonal
Gene Full Name Actin, cytoplasmic 1
Alias Symbols BRWS1, PS1TP5BP1
Product Format Lyophilized from 1.2% sodium acetate, with 2 mg/ml BSA and 0.01 mg sodium azide
Reference 1. Ng, S.-Y.; Gunning, P.; Eddy, R.; Ponte, P.; Leavitt, J.; Kedes, L.; Shows, T. : Chromosome 7 assignment of the human beta-actin functional gene (ACTB) and the chromosomal dispersion of pseudogenes. (Abstract) Cytogenet. Cell Genet. 40: 712 only, 1985.
2. Procaccio, V.; Salazar, G.; Ono, S.; Styers, M. L.; Gearing, M.; Davila, A.; Jimenez, R.; Juncos, J.; Gutekunst, C.-A.; Meroni, G.; Fontanella, B.; Sontag, E.; Sontag, J. M.; Faundez, V.; Wainer, B. H. : A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia. Am. J. Hum. Genet. 78: 947-960, 2006.
3. Ng, S.-Y.; Gunning, P.; Eddy, R.; Ponte, P.; Leavitt, J.; Shows, T.; Kedes, L. : Evolution of the functional human beta-actin gene and its multi-pseudogene family: conservation of the noncoding regions and chromosomal dispersion of pseudogenes. Molec. Cell. Biol. 5: 2720-2732, 1985.
Description of Target The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al. (1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.
Reconstitution and Storage Reconstitute with 1.2% sodium acetate or neutral PBS. If 1 mL of PBS is used, the antibody concentration will be 100 ug/mL. Store at -20°C for one year. After reconstitution, at 4°C for three months. It can also be aliquotted and stored frozen at -20°C for a longer time.
Datasheets/Manuals Printable datasheet for anti-ACTB (OAAD00224) antibody
Specificity Human, mouse, rat.
No cross reactivity with other proteins.
Application Info WB: 0.25 - 0.5 ug/mL with the appropriate system to detect beta-actin in cells and tissues
IHC-P: 0.5 - 1 ug/mL to detect beta-actin in formalin fixed and paraffin embedded tissues.
Immunogen Slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
Uniprot ID P60709
Protein Name Actin, cytoplasmic 1
Publications

Selective cytoprotective effect of histamine on doxorubicin-induced hepatic and cardiac toxicity in animal models. Cell Death Discov. 1, 15059 (2015). 27551485

Protein Accession # NP_001092.1
Purification Purified by the goat anti-mouse IgG Affinity chromatography.
Nucleotide Accession # NM_001101.3
Gene Symbol ACTB
Predicted Species Reactivity Human, Mouse, Rat
Application WB, IHC, IP
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