| Product Number |
OAAD00212 |
| Product Page |
www.avivasysbio.com/vimentin-antibody-oaad00212.html |
| Name |
VIMENTIN Antibody (OAAD00212) |
| Clone |
VMT-24 |
| Isotype |
IgG1 |
| NCBI Gene Id |
7431 |
| Host |
Mouse |
| Clonality |
Monoclonal |
| Concentration |
0.5 mg/ml |
| Alias Symbols |
HEL113, CTRCT30, |
| Product Format |
Lyophilized from 1.2% sodium acetate, with 2 mg BSA and 0.01 mg sodium azide |
| Reference |
1. Lilienbaum, A.; Legagneux, V.; Portier, M.-M.; Dellagi, K.; Paulin, D. : Vimentin gene: expression in human lymphocytes and in Burkitt's lymphoma cells. EMBO J. 5: 2809-2814, 1986.
2. Mor-Vaknin, N.; Punturieri, A.; Sitwala, K.; Markovitz, D. M. : Vimentin is secreted by activated macrophages. Nature Cell Biol. 5: 59-63, 2003 3 Ferrari, S.; Cannizzaro, L. A.; Battini, R.; Huebner, K.; Baserga, R. : The gene encoding human vimentin is located on the short arm of chromosome 10. Am. J. Hum. Genet. 41: 616-626, 1987. |
| Description of Target |
The VIM gene was one of many that Gieser and Swaroop (1992) recovered from a subtracted cDNA library for retinal pigment epithelium. Vimentin gene express in human lymphocytes and in Burkitt's lymphoma cells. Vimentin is secreted by activated macrophages. The gene encoding human vimentin is located on the short arm of chromosome 10. |
| Reconstitution and Storage |
Store at -20°C for one year. Reconstitute with 1.2% sodium acetate or neutral PBS. If 1 mL of PBS is used, the antibody concentration will be 100 ug/mL. After reconstitution, store at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. |
| Datasheets/Manuals |
Printable datasheet for VIMENTIN Antibody (OAAD00212) - OAAD00212 |
| Specificity |
Human, rat.
No cross reactivity with other proteins. |
| Application Info |
WB: 0.5-1 ug/mL with the appropriate system to detect vimentin in cells and tissues
IHC-P: 1.5-2.5 ug/mL to detect vimentin in formalin fixed and paraffin embedded tissues. |
| Immunogen |
Pig eye lens vimentin. |
| Storage Buffer |
1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml. |
| Uniprot ID |
P08670 |
| Protein Name |
Vimentin |
| Purification |
Purified by the goat anti-mouse IgG Affinity chromatography. |
| Gene Symbol |
VIM |
| Predicted Species Reactivity |
Human, Rat |
| Application |
WB, IHC, IP |
| Image 1 | Peptide
 | Western blot At 0.5-1?g/ml with the appropriate system to detect vimentin in cells and tissues. |
|
|
