Product Number |
OAAD00191 |
Product Page |
www.avivasysbio.com/paxillin-antibody-oaad00191.html |
Name |
PAXILLIN Antibody (OAAD00191) |
Clone |
PAX-14 |
Isotype |
IgG1 |
NCBI Gene Id |
5829 |
Host |
Mouse |
Clonality |
Monoclonal |
Reference |
1 Salgia, R.; Li, J.-L.; Lo, S. H.; Brunkhorst, B.; Kansas, G. S.; Sobhany, E. S.; Sun, Y.; Pisick, E.; Hallek, M.; Ernst, T.; Tantravahi, R.; Chen, L. B.; Griffin, J. D. : Molecular cloning of human paxillin, a focal adhesion protein phosphorylated by P210(BCR/ABL). J. Biol. Chem. 270: 5039-5047, 1995. 2. Turner, C. E.; Glenney, J. R., Jr.; Burridge, K. : Paxillin: a new vinculin-binding protein present in focal adhesions. J. Cell Biol. 111: 1059-1068, 1990. |
Description of Target |
The paxillin gene can be alternatively spliced to include 1 of 2 alternative exons, generating beta and gamma isoforms. Paxillin is a 68-kDa focal adhesion protein that is phosphorylated on tyrosine residues in fibroblasts in response to transformation by v-src, treatment with platelet-derived growth factor, or cross-linking of integrins. The 68-kD protein (paxillin) is a cytoskeletal component that localizes to the focal adhesions at the ends of actin stress fibers in chicken embryo fibroblasts. It is also present in the focal adhesions of Madin-Darby bovine kidney (MDBK) epithelial cells but is absent, like talin, from the cell-cell adherens junctions of these cells. |
Reconstitution and Storage |
At -20C for one year. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for longer time. |
Datasheets/Manuals |
Printable datasheet for PAXILLIN Antibody (OAAD00191) - OAAD00191 |
Specificity |
Human, mouse, rat, chicken No cross reactivity with other proteins. |
Application Info |
Western blot At 1-2ug/ml with the appropriate system to detect paxillin in cells and tissues. Immunocytochemistry Suitable Other applications have not been tested. Optimal dilutions should be determined by end user. |
Immunogen |
C-terminal part of recombinant chicken paxillin (amino acids 305-559) |
Formulation |
Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.01mg NaN3 as preservative. |
Storage Buffer |
1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml. |
Uniprot ID |
P49023 |
Protein Name |
Paxillin |
Purification |
Purified by the goat anti-mouse IgG Affinity chromatography. |
Gene Symbol |
PXN |
Predicted Species Reactivity |
Human, Mouse, Rat, Chicken |
Application |
WB, IHC, ICC |
Image 1 | |
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