PAXILLIN Antibody (OAAD00191)

Data Sheet
 
Product Number OAAD00191
Product Page www.avivasysbio.com/paxillin-antibody-oaad00191.html
Name PAXILLIN Antibody (OAAD00191)
Clone PAX-14
Isotype IgG1
NCBI Gene Id 5829
Host Mouse
Clonality Monoclonal
Reference 1 Salgia, R.; Li, J.-L.; Lo, S. H.; Brunkhorst, B.; Kansas, G. S.; Sobhany, E. S.; Sun, Y.; Pisick, E.; Hallek, M.; Ernst, T.; Tantravahi, R.; Chen, L. B.; Griffin, J. D. : Molecular cloning of human paxillin, a focal adhesion protein phosphorylated by P210(BCR/ABL). J. Biol. Chem. 270: 5039-5047, 1995.
2. Turner, C. E.; Glenney, J. R., Jr.; Burridge, K. : Paxillin: a new vinculin-binding protein present in focal adhesions. J. Cell Biol. 111: 1059-1068, 1990.
Description of Target The paxillin gene can be alternatively spliced to include 1 of 2 alternative exons, generating beta and gamma isoforms. Paxillin is a 68-kDa focal adhesion protein that is phosphorylated on tyrosine residues in fibroblasts in response to transformation by v-src, treatment with platelet-derived growth factor, or cross-linking of integrins. The 68-kD protein (paxillin) is a cytoskeletal component that localizes to the focal adhesions at the ends of actin stress fibers in chicken embryo fibroblasts. It is also present in the focal adhesions of Madin-Darby bovine kidney (MDBK) epithelial cells but is absent, like talin, from the cell-cell adherens junctions of these cells.
Reconstitution and Storage At -20C for one year. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for longer time.
Datasheets/Manuals Printable datasheet for PAXILLIN Antibody (OAAD00191) - OAAD00191
Specificity Human, mouse, rat, chicken No cross reactivity with other proteins.
Application Info Western blot At 1-2ug/ml with the appropriate system to detect paxillin in cells and tissues.
Immunocytochemistry Suitable Other applications have not been tested.
Optimal dilutions should be determined by end user.
Immunogen C-terminal part of recombinant chicken paxillin (amino acids 305-559)
Formulation Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.01mg NaN3 as preservative.
Storage Buffer 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.
Uniprot ID P49023
Protein Name Paxillin
Purification Purified by the goat anti-mouse IgG Affinity chromatography.
Gene Symbol PXN
Predicted Species Reactivity Human, Mouse, Rat, Chicken
Application WB, IHC, ICC
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