CCND1 Antibody (OAAD00148)

Data Sheet
 
Product Number OAAD00148
Product Page www.avivasysbio.com/ccnd1-antibody-oaad00148.html
Name CCND1 Antibody (OAAD00148)
Clone CY-D1
Isotype IgG2a
NCBI Gene Id 595
Host Mouse
Clonality Monoclonal
Gene Full Name G1/S-specific cyclin-D1
Alias Symbols BCL1, PRAD1, U21B31, D11S287E
Reference 1. Rosenberg, C. L.; Kim, H. G.; Shows, T. B.; Kronenberg, H. M.; Arnold, A. : Rearrangement and overexpression of D11S287E, a candidate oncogene on chromosome 11q13 in benign parathyroid tumors. Oncogene 6: 449-453, 1991.
2. Muller, H.; Lukas, J.; Schneider, A.; Warthoe, P.; Bartek, J.; Eilers, M.; Strauss, M. : Cyclin D1 expression is regulated by the retinoblastoma protein. Proc. Nat. Acad. Sci. 91: 2945-2949, 1994.
3. Hinds, P. W.; Dowdy, S. F.; Eaton, E. N.; Arnold, A.; Weinberg, R. A. : Function of a human cyclin gene as an oncogene. Proc. Nat. Acad. Sci. 91: 709-713, 1994.
Description of Target D-type cyclins (cyclins D1, D2, and D3) are regarded as essential links between cell environment and the core cell cycle machinery. CyclinD1(CCND1), also known as BCL1. Expression of cyclin D1, but not of cyclins A and E, was induced by transfection of the RB gene into RB-deficient tumor cells. CCND1 gene to 11q13. cyclin D1 gene can function as an oncogene. Cyclin D1 expression is regulated by the retinoblastoma protein.
Reconstitution and Storage At -20C for one year. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for longer time.
Datasheets/Manuals Printable datasheet for anti-CCND1 (OAAD00148) antibody
Specificity Human, mouse, rat.
No cross reactivity with other proteins.
Application Info Western blot At 4ug/ml with the appropriate system to detect cyclin D1 in cells and tissues.
Immunohistochemistry(P) At 8ug/ml to detect cyclin D1 in formalin fixed and paraffin embedded tissues.
Immunohistochemistry(F) At 8ug/ml to detect cyclin D1 in formalin or acetone fixed tissues.
Immunocytochemistry Suitable Other applications have not been tested.
Optimal dilutions should be determined by end user.
Immunogen Recombinant human cyclin D1 protein.
Formulation Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.01mg NaN3 as preservative.
Storage Buffer 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.
Uniprot ID P24385
Protein Name G1/S-specific cyclin-D1
Protein Accession # NP_444284.1
Purification Purified by the goat anti-mouse IgG Affinity chromatography.
Nucleotide Accession # NM_053056.2
Gene Symbol CCND1
Predicted Species Reactivity Human, Mouse, Rat
Application WB, IHC, IP, ICC
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