SEMA4D Antibody - C-terminal region (OAAB19368)

• Product Number: OAAB19368
• Name: SEMA4D Antibody - C-terminal region (OAAB19368)
• Molecular Weight: 96 kDa
• Isotype: Rabbit Ig
• NCBI Gene Id: 10507
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Alias Symbols: A8, GR3, BB18, CD100, COLL4, SEMAJ, coll-4, C9orf164, M-sema-G
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Reference: Hall K.T.,et al.Proc. Natl. Acad. Sci. U.S.A. 93:11780-11785(1996).
  Humphray S.J.,et al.Nature 429:369-374(2004).
  Ota T.,et al.Nat. Genet. 36:40-45(2004).
  Tamagnone L.,et al.Cell 99:71-80(1999).
  Basile J.R.,et al.Mol. Cell. Biol. 25:6889-6898(2005).
  
• Description of Target: Cell surface receptor for PLXN1B and PLXNB2 that plays an important role in cell-cell signaling. Promotes reorganization of the actin cytoskeleton and plays a role in axonal growth cone guidance in the developing central nervous system. Regulates dendrite and axon branching and morphogenesis. Promotes the migration of cerebellar granule cells and of endothelial cells. Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro). Promotes signaling via SRC and PTK2B/PYK2, which then mediates activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Interaction with PLXNB1 mediates activation of RHOA.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Cellular Location: Cell membrane; Single-pass type I membrane protein.
  Tissue Location: Strongly expressed in skeletal muscle, peripheral blood lymphocytes, spleen, and thymus and also expressed at lower levels in testes, brain, kidney, small intestine, prostate, heart, placenta, lung and pancreas, but not in colon and liver.
• Application Info: WB: 1:2000
• Immunogen: This SEMA4D antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 793-832 amino acids from the C-terminal region of human SEMA4D.
• Uniprot ID: Q92854
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Gene Symbol: SEMA4D
• Predicted Species Reactivity: Human
• Application: WB
• Image 1: Jurkat whole cell lysates, HL-60 whole cell lysates
  
  All lanes: Anti-SEMA4D Antibody (C-term) at 1: 2000 dilution
  Lane 1: Jurkat whole cell lysates
  Lane 2: HL-60 whole cell lysates
  Lysates/proteins at 20 ug per lane.
  Secondary
  Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
  Predicted band size: 96 kDa
  Blocking/Dilution buffer: 5% NFDM/TBST.