CSF1R Antibody (OAAB18690)

• Product Number: OAAB18690
• Name: CSF1R Antibody (OAAB18690)
• Molecular Weight: 108 kDa
• Clone: 1486CT328.53.37
• Isotype: IgG1, k
• NCBI Gene Id: 1436
• Host: Mouse
• Clonality: Monoclonal
• Concentration: 0.5 mg/ml
• Description:
  View product citations for CSF1R Antibody (OAAB18690) on CiteAb
• Alias Symbols: FMS, CSFR, FIM2, HDLS, C-FMS, CD115, CSF-1R, BANDDOS, M-CSF-R
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Reference: Hampe A.,et al.Oncogene Res. 4:9-17(1989).
  Coussens L.,et al.Nature 320:277-280(1986).
  Andre C.,et al.Genomics 39:216-226(1997).
  Jin P.,et al.Arthritis Res. Ther. 10:R73-R73(2008).
  Schmutz J.,et al.Nature 431:268-274(2004).
  
• Description of Target: Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding. Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP- 1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor.
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Cellular Location: Cell membrane; Single-pass type I membrane protein.
  Tissue Location: Expressed in bone marrow and in differentiated blood mononuclear cells.
• Application Info: IHC-P: 1:25
  FC: 1:25
  WB: 1:4000
• Immunogen: This CSF1R antibody is generated from a mouse immunized with a recombinant protein.
• Uniprot ID: P07333
• Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.
• Gene Symbol: CSF1R
• Predicted Species Reactivity: Human
• Application: IHC-P, FC, WB, ELISA
• Image 1: Human placenta lysates
  
  Anti-CSF1R Antibodyat 1: 2000 dilution + human placenta lysates
  Lysates/proteins at 20 ug per lane.
  Secondary
  Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
  Predicted band size: 108 kDa
  Blocking/Dilution buffer: 5% NFDM/TBST.
• Image 2: U-87MG whole cell lysates
  
  Anti-CSF1R Antibodyat 1: 4000 dilution + U-87MG whole cell lysates
  Lysates/proteins at 20 ug per lane.
  Secondary
  Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
  Predicted band size: 108 kDa
  Blocking/Dilution buffer: 5% NFDM/TBST.