| Product Number |
OAAB18687 |
| Product Page |
www.avivasysbio.com/fhit-antibody-oaab18687.html |
| Name |
FHIT Antibody (OAAB18687) |
| Molecular Weight |
17 kDa |
| Clone |
1093CT23.1.2 |
| Isotype |
IgG1, k |
| NCBI Gene Id |
2272 |
| Host |
Mouse |
| Clonality |
Monoclonal |
| Alias Symbols |
FRA3B, AP3Aase |
| Product Format |
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. |
| Reference |
Ohta M.,et al.Cell 84:587-597(1996).
Druck T.,et al.Cancer Res. 57:504-512(1997).
Corominas R.,et al.Nat. Commun. 5:3650-3650(2014).
Naqvi S.R.A.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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| Description of Target |
Cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P(1)-P(4)-bis(5'- adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP. Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5. Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways. Inhibits MDM2-mediated proteasomal degradation of p53/TP53 and thereby plays a role in p53/TP53-mediated apoptosis. Induction of apoptosis depends on the ability of FHIT to bind P(1)-P(3)-bis(5'-adenosyl) triphosphate or related compounds, but does not require its catalytic activity, it may in part come from the mitochondrial form, which sensitizes the low- affinity Ca(2+) transporters, enhancing mitochondrial calcium uptake. Functions as tumor suppressor. |
| Reconstitution and Storage |
Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-FHIT (OAAB18687) antibody |
| Application Info |
FC: 1:25
WB: 1:4000 |
| Immunogen |
This FHIT antibody is generated from a mouse immunized with a recombinant protein. |
| Uniprot ID |
P49789 |
| Purification |
This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Gene Symbol |
FHIT |
| Predicted Species Reactivity |
Human |
| Application |
WB |
| Image 1 | HL-60 whole cell lysates, MDA-MB-453 whole cell lysates
 | All lanes: Anti-FHIT Antibody at 1: 4000 dilution
Lane 1: HL-60 whole cell lysates
Lane 2: MDA-MB-453 whole cell lysates
Lysates/proteins at 20 ug per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size: 17 kDa
Blocking/Dilution buffer: 5% NFDM/TBST. |
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