PDK2 Antibody (OAAB17672)

• Product Number: OAAB17672
• Name: PDK2 Antibody (OAAB17672)
• Molecular Weight: 46154 Da
• Isotype: Ig
• NCBI Gene Id: 5164
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: PDHK2, PDKII
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: Serine/threonine kinase that plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism. Mediates cellular responses to insulin. Plays an important role in maintaining normal blood glucose levels and in metabolic adaptation to nutrient availability. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. Plays a role in the regulation of cell proliferation and in resistance to apoptosis under oxidative stress. Plays a role in p53/TP53-mediated apoptosis
• Application Info: WB: 1:1000
  FC: 1:10~50
  IHC-P: 1:10~50
• Immunogen: This PDK2 antibody is generated from rabbits immunized with recombinant protein of human PDK2.
• Additional Information: Cellular Location: Mitochondrion matrix.
  Tissue Location: Expressed in many tissues, with the highest level in heart and skeletal muscle, intermediate levels in brain, kidney, pancreas and liver, and low levels in placenta and lung
• Intended Use: PDK2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
• Uniprot ID: Q15119
• Protein Name: [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2, mitochondrial
• Protein Accession #: NP_001186827.1
• Gene Symbol: PDK2
• Predicted Species Reactivity: Human
• Application: WB, FC, IHC
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: Jurkat cell
  
  PDK2 Antibody (Cat. #OAAB17672) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Image 2: skeletal muscle
  
  PDK2 Antibody (Cat. #OAAB17672) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PDK2 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
• Image 3: Jurkat cell
  
  Western blot analysis of PDK2 Antibody (Cat. #OAAB17672) in Jurkat cell line lysates (35ug/lane). PDK2 (arrow) was detected using the purified Pab.