PP2A alpha Antibody - N - terminal region (OAAB17633)

• Product Number: OAAB17633
• Name: PP2A alpha Antibody - N - terminal region (OAAB17633)
• Molecular Weight: 35594 Da
• Isotype: Ig
• NCBI Gene Id: 5515
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: RP-C, PP2Ac, PP2CA, NEDLBA, PP2Calpha
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
• Immunogen: This PP2A alpha antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PP2A alpha.
• Additional Information: Cellular Location: Cytoplasm. Nucleus. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle pole. Note=In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2 (By similarity)
• Intended Use: PP2A alpha Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Uniprot ID: P67775
• Protein Name: Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
• Protein Accession #: NP_002706.1
• Gene Symbol: PPP2CA
• Predicted Species Reactivity: Human
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: A2058
  
  Western blot analysis of anti-hPPP2CA/B Antibody (N-term) (Cat.# OAAB17633) in A2058 cell line lysates (35ug/lane). PPP2CA/B (arrow) was detected using the purified Pab.
• Image 2: human breast carcinoma
  
  Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.