PPM1F Antibody - N-terminal region (OAAB17629)

Data Sheet
 
Product Number OAAB17629
Product Page www.avivasysbio.com/ppm1f-antibody-n-terminal-region-oaab17629.html
Name PPM1F Antibody - N-terminal region (OAAB17629)
Molecular Weight 49831 Da
Isotype Ig
NCBI Gene Id 9647
Host Rabbit
Clonality Polyclonal
Concentration Approximately 0.5mg/ml. Actual concentration varies with each lot.
Alias Symbols CAMKP, FEM-2, POPX2, hFEM-2, CaMKPase
Product Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Description of Target Purified Rabbit Polyclonal Antibody (Pab)
Reconstitution and Storage Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for OAAB17629
Additional Information Precautions: PPM1F Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Additional Information Function: Dephosphorylates and concomitantly deactivates CaM- kinase II activated upon autophosphorylation, and CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase Promotes apoptosis
Application Info WB: 1:1000
IHC-P: 1:50~100
Immunogen This PPM1F antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11-40 amino acids from the N-terminal region of human PPM1F.
Uniprot ID P49593
Protein Name Protein phosphatase 1F
Sample Type Confirmation

PPM1F is strongly supported by BioGPS gene expression data to be expressed in HEK293

Protein Accession # NP_055449.1
Gene Symbol PPM1F
Predicted Species Reactivity Human
Application WB, IHC
Predicted Homology Based on Immunogen Sequence Human
Image 1
breast carcinoma
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Image 2
Mouse Liver
Western blot analysis of anti-PPM1F Pab (Cat. # OAAB17629) in mouse liver tissue lysate (35ug/lane). PPM1F(arrow) was detected using the purified Pab.
Image 3
293
Western blot analysis of PPM1F (arrow) using PPM1F Antibody (N-term) (Cat.# OAAB17629). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PPM1F gene (Lane 2) (Origene Technologies).

PPM1F is strongly supported by BioGPS gene expression data to be expressed in HEK293

 

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