GBL Antibody - middle region (OAAB17572)

• Product Number: OAAB17572
• Name: GBL Antibody - middle region (OAAB17572)
• Molecular Weight: 35876 Da
• Isotype: Ig
• NCBI Gene Id: 64223
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: GBL, LST8, POP3, WAT1, GbetaL
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: GBL Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals mTORC1 is activated in response to growth factors or amino acids Growth factor-stimulated mTORC1 activation involves a AKT1- mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with MTOR and enhances its kinase activity. In nutrient- poor conditions, stabilizes the MTOR-RPTOR interaction and favors RPTOR-mediated inhibition of MTOR activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum- induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
• Immunogen: This GBL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140-170 amino acids from the Central region of human GBL.
• Additional Information: Cellular Location: Cytoplasm (By similarity).
  Tissue Location: Broadly expressed, with highest levels in skeletal muscle, heart and kidney
• Uniprot ID: Q9BVC4
• Protein Name: Target of rapamycin complex subunit LST8
• Sample Type Confirmation:

  MLST8 is strongly supported by BioGPS gene expression data to be expressed in HEK293

• Protein Accession #: NP_001186102.1
• Gene Symbol: MLST8
• Predicted Species Reactivity: Human
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: 293, GBL
  
  Western blot analysis of GBL Antibody (Center) polyclonal antibody(Cat.# OAAB17572) (arrow). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GBL gene (Lane 2) (Origene Technologies).

  MLST8 is strongly supported by BioGPS gene expression data to be expressed in HEK293

• Image 2: Human breast carcinoma
  
  Formalin-fixed and paraffin-embedded human breast carcinoma reacted with GBL antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.