BRSK1 Antibody - C-terminal region (OAAB17558)

• Product Number: OAAB17558
• Name: BRSK1 Antibody - C-terminal region (OAAB17558)
• Molecular Weight: 85087 Da
• Isotype: Ig
• NCBI Gene Id: 84446
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: hSAD1
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: BRSK1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Serine/threonine-protein kinase that plays a key role in polarization of neurons and centrosome duplication. Phosphorylates CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-529' and 'Ser-579'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in post-mitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. In neurons, localizes to synaptic vesicles and plays a role in neurotransmitter release, possibly by phosphorylating RIMS1. Also acts as a positive regulator of centrosome duplication by mediating phosphorylation of gamma- tubulin (TUBG1 and TUBG2) at 'Ser-131', leading to translocation of gamma-tubulin and its associated proteins to the centrosome Involved in the UV-induced DNA damage checkpoint response, probably by inhibiting CDK1 activity through phosphorylation and activation of WEE1, and inhibition of CDC25B and CDC25C
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
• Immunogen: This BRSK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 654-684 amino acids from the C-terminal region of human BRSK1.
• Additional Information: Cellular Location: Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, centrosome (By similarity). Cell junction, synapse (By similarity). Note=Localizes to synaptic vesicles in neurons (By similarity). Nuclear in the absence of DNA damage. Translocated to the nucleus in response to UV- or MMS-induced DNA damage
  Tissue Location: Widely expressed, with highest levels in brain and testis. Protein levels remain constant throughout the cell cycle
• Uniprot ID: Q8TDC3
• Protein Name: Serine/threonine-protein kinase BRSK1
• Protein Accession #: NP_115806.1
• Gene Symbol: BRSK1
• Predicted Species Reactivity: Human
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: Mouse Liver
  
  KIAA1811 Antibody (S685) (Cat. # OAAB17558) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the KIAA1811 antibody detected the KIAA1811 protein (arrow).
• Image 2: Human Testis
  
  BRSK1 Antibody (C-term) (Cat. # OAAB17558) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BRSK1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.