YWHAB Antibody - C-terminal region (OAAB17541)

Data Sheet
 
Product Number OAAB17541
Product Page www.avivasysbio.com/ywhab-antibody-c-terminal-region-oaab17541.html
Name YWHAB Antibody - C-terminal region (OAAB17541)
Molecular Weight 28082 Da
Isotype Ig
NCBI Gene Id 7529
Host Rabbit
Clonality Polyclonal
Concentration Approximately 0.5mg/ml. Actual concentration varies with each lot.
Alias Symbols HS1, GW128, YWHAA, KCIP-1, HEL-S-1
Product Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Description of Target Purified Rabbit Polyclonal Antibody (Pab)
Reconstitution and Storage Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for OAAB17541
Additional Information Precautions: YWHAB Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Additional Information Function: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2
Application Info IHC-P: 1:50~100
WB: 1:1000
IF: 1:10~50
Immunogen This YWHAB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217-246 amino acids from the C-terminal region of human YWHAB.
Additional Information Cellular Location: Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
Uniprot ID P31946
Protein Name 14-3-3 protein beta/alpha
Sample Type Confirmation

There is BioGPS gene expression data showing that YWHAB is expressed in HEK293, Jurkat

Protein Accession # NP_003395.1
Gene Symbol YWHAB
Predicted Species Reactivity Human
Application IHC, WB, IF
Predicted Homology Based on Immunogen Sequence Human
Image 1
breast carcinoma
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Image 2
Hela, Nuclear
Confocal immunofluorescent analysis of YWHAB Antibody (C-term) (Cat# OAAB17541) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Image 3
Jurkat
The anti-YWHAB Pab (Cat. # OAAB17541) is used in Western blot to detect YWHAB in Jurkat cell lysate.
Image 4
293
Western blot analysis of YWHAB (arrow) using rabbit polyclonal YWHAB Antibody (C-term) (Cat. # OAAB17541). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the YWHAB gene.

There is BioGPS gene expression data showing that YWHAB is expressed in HEK293, Jurkat

 

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