DGKQ Antibody - C-terminal region (OAAB17482)

• Product Number: OAAB17482
• Name: DGKQ Antibody - C-terminal region (OAAB17482)
• Molecular Weight: 101155 Da
• Isotype: Ig
• NCBI Gene Id: 1609
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: DAGK, DAGK4, DAGK7
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: DGKQ Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). May regulate the activity of protein kinase C by controlling the balance between these two signaling lipids. Activated in the nucleus in response to alpha-thrombin and nerve growth factor (By similarity). May be involved in cAMP- induced activation of NR5A1 and subsequent steroidogenic gene transcription by delivering PA as ligand for NR5A1. Acts synergistically with NR5A1 on CYP17 transcriptional activity
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  FC: 1:10~50
• Immunogen: This DGKQ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 912-942 amino acids from the C-terminal region of human DGKQ.
• Additional Information: Cellular Location: Cytoplasm. Cell membrane. Cytoplasm, cytoskeleton. Nucleus. Nucleus speckle. Note=Translocates to the nucleus in response to thrombin stimulation (By similarity) Translocates to the plasma membrane in response to steroid hormone receptor stimulation. Translocation to the plasma membrane is dependent on G-protein coupled receptor stimulation and subsequent activation of PRKCE and probably PRKCH
• Uniprot ID: P52824
• Protein Name: Diacylglycerol kinase theta
• Sample Type Confirmation:

  DGKQ is strongly supported by BioGPS gene expression data to be expressed in NCI-H460

• Protein Accession #: NP_001338.2
• Gene Symbol: DGKQ
• Predicted Species Reactivity: Human
• Application: WB, IHC, FC
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: NCI-H460
  
  DGKQ Antibody (R927) (Cat.# OAAB17482) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the DGKQ antibody detected the DGKQ protein (arrow).

  DGKQ is strongly supported by BioGPS gene expression data to be expressed in NCI-H460

• Image 2: Human Brain
  
  DGKQ Antibody (C-term) (Cat. # OAAB17482) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DGKQ Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
• Image 3: NCI-H460
  
  DGKQ Antibody (C-term) (Cat. # OAAB17482) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.