WEE1 Antibody - C-terminal region (OAAB17471)

• Product Number: OAAB17471
• Name: WEE1 Antibody - C-terminal region (OAAB17471)
• Molecular Weight: 71597 Da
• Isotype: Ig
• NCBI Gene Id: 7465
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: WEE1A, WEE1hu
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: WEE1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
• Immunogen: This WEE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 604-634 amino acids from the C-terminal region of human WEE1.
• Additional Information: Cellular Location: Nucleus.
• Uniprot ID: P30291
• Protein Name: Wee1-like protein kinase
• Sample Type Confirmation:

  WEE1 is supported by BioGPS gene expression data to be expressed in HL60

• Protein Accession #: NP_001137448.1
• Gene Symbol: WEE1
• Predicted Species Reactivity: Human
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: HL-60
  
  Western blot analysis of anti-WEE1 Pab (Cat. # OAAB17471) in HL-60 cell lysate. WEE1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

  WEE1 is supported by BioGPS gene expression data to be expressed in HL60

• Image 2: hepatocarcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.