Protein Kinase A regulatory subunit I alpha Antibody - N-terminal region (OAAB17462)

• Product Number: OAAB17462
• Name: Protein Kinase A regulatory subunit I alpha Antibody - N-terminal region (OAAB17462)
• Molecular Weight: 42982 Da
• Isotype: Ig
• NCBI Gene Id: 5573
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: CAR, CNC, CNC1, PKR1, TSE1, ADOHR, PPNAD1, PRKAR1, ACRDYS1
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: Protein Kinase A regulatory subunit I alpha Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
• Immunogen: This Protein Kinase A regulatory subunit I alpha antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human Protein Kinase A regulatory subunit I alpha.
• Additional Information: Cellular Location: Cell membrane.
  Tissue Location: Four types of regulatory chains are found: I- alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible
• Uniprot ID: P10644
• Protein Name: cAMP-dependent protein kinase type I-alpha regulatory subunit
• Sample Type Confirmation:

  There is BioGPS gene expression data showing that PRKAR1A is expressed in HEK293

• Protein Accession #: NP_001263218.1
• Gene Symbol: PRKAR1A
• Predicted Species Reactivity: Human
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: hepatocarcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
• Image 2: 293
  
  Western blot analysis of PKR1 (arrow) using rabbit polyclonal hPKR1-M1 (Cat. # OAAB17462). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PKR1 gene (Lane 2).(2ug/ml)
• Image 3: CEM
  
  Western blot analysis of anti-PKR1 Antibody (N-term) (Cat.# OAAB17462) in CEM cell line lysates (35ug/lane). PKR1 (arrow) was detected using the purified Pab.

  There is BioGPS gene expression data showing that PRKAR1A is expressed in HEK293