NM23 Antibody - N-terminal region (OAAB17441)

• Product Number: OAAB17441
• Name: NM23 Antibody - N-terminal region (OAAB17441)
• Molecular Weight: 17149 Da
• Isotype: Ig
• NCBI Gene Id: 4830
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: NB, AWD, NBS, GAAD, NDKA, NM23, NDPKA, NDPK-A, NM23-H1
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: NM23 (NME1) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Major role in the synthesis of nucleoside triphosphates other than ATP. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein- coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination
• Application Info: WB: 1:1000
  IF: 1:10~50
  IHC-P: 1:50~100
• Immunogen: This NM23 (NME1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-54 amino acids from the N-terminal region of human NM23 (NME1).
• Additional Information: Cellular Location: Cytoplasm. Nucleus. Note=Cell-cycle dependent nuclear localization which can be induced by interaction with Epstein-barr viral proteins or by degradation of the SET complex by GzmA
  Tissue Location: Isoform 1 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen and thymus. Expressed in lung carcinoma cell lines but not in normal lung tissues. Isoform 2 is ubiquitously expressed and its expression is also related to tumor differentiation. Isoform 3 is ubiquitously expressed
• Uniprot ID: P15531
• Protein Name: Nucleoside diphosphate kinase A
• Sample Type Confirmation:

  NME1 is strongly supported by BioGPS gene expression data to be expressed in HEK293

• Protein Accession #: NP_000260.1
• Gene Symbol: NME1
• Predicted Species Reactivity: Human
• Application: WB, IF
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: human cancer tissue
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
• Image 2: 293 cell
  
  NME1 Antibody (F40) (Cat. #OAAB17441) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the NME1 antibody detected the NME1 protein (arrow).
• Image 3: A375 cell
  
  Confocal immunofluorescent analysis of NM23 (NME1) Antibody (N-term)(Cat#OAAB17441) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).