DGKZ Antibody - C-terminal region (OAAB17418)

• Product Number: OAAB17418
• Name: DGKZ Antibody - C-terminal region (OAAB17418)
• Molecular Weight: 124128 Da
• Isotype: Ig
• NCBI Gene Id: 8525
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: DAGK5, DAGK6, DGK-ZETA, hDGKzeta
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: DGKZ Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Displays a strong preference for 1,2-diacylglycerols over 1,3-diacylglycerols, but lacks substrate specificity among molecular species of long chain diacylglycerols. Isoform 2 but not isoform 1 regulates RASGRP1 activity
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  IF: 1:10~50
• Immunogen: This DGKZ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 989-1019 amino acids from the C-terminal region of human DGKZ.
• Additional Information: Cellular Location: Cytoplasm. Nucleus. Cell membrane.
  Tissue Location: Highest levels in brain, and substantial levels in skeletal muscle, heart, and pancreas. Isoform 1 is predominantly expressed in muscle
• Uniprot ID: Q13574
• Protein Name: Diacylglycerol kinase zeta
• Protein Accession #: NP_001099010.1
• Gene Symbol: DGKZ
• Predicted Species Reactivity: Human
• Application: WB, IHC, IF
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: H295R cell
  
  SF1 colocalizes with DGK and PA in the nuclei of H295R cells. Cells were plated onto glass coverslips, fixed, permeabilized, and incubated with anti-SF1 and anti-DGKZ (B) for 1 h. Coverslips were washed and incubated with anti-fluorescein isothiocyanate and antirhodamine, and immunofluorescence was detected by confocal microscopy.
• Image 2: 293 cell
  
  Western blot analysis of DGKZ (arrow) using DGKZ Antibody (C-term) (Cat.#OAAB17418). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DGKZ gene (Lane 2) (Origene Technologies).
• Image 3: Ramos cell
  
  DGKZ Antibody (D1004) (Cat. #OAAB17418) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the DGKZ antibody detected the DGKZ protein (arrow).
• Image 4: human cancer tissue
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.