| Product Number |
OAAB17416 |
| Product Page |
www.avivasysbio.com/bubr1-bub1b-antibody-n-terminal-region-oaab17416.html |
| Name |
BUBR1 Antibody - N-terminal region (OAAB17416) |
| Molecular Weight |
119545 Da |
| Isotype |
Ig |
| NCBI Gene Id |
701 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
Approximately 0.5mg/ml. Actual concentration varies with each lot. |
| Alias Symbols |
MVA1, SSK1, BUBR1, Bub1A, MAD3L, hBUBR1, BUB1beta |
| Product Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Description of Target |
Purified Rabbit Polyclonal Antibody (Pab) |
| Reconstitution and Storage |
Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for OAAB17416 |
| Additional Information |
Precautions: BUBR1 (BUB1B) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Additional Information |
Function: Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression |
| Application Info |
WB: 1:1000
IHC-P: 1:50~100 |
| Immunogen |
This BUBR1 (BUB1B) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3-33 amino acids from the N-terminal region of human BUBR1 (BUB1B). |
| Additional Information |
Cellular Location: Cytoplasm. Nucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, centrosome. Note=Cytoplasmic in interphase cells. Associates with the kinetochores in early prophase. Kinetochore localization requires BUB1, PLK1 and CASC5
Tissue Location: Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index |
| Uniprot ID |
O60566 |
| Protein Name |
Mitotic checkpoint serine/threonine-protein kinase BUB1 beta |
| Sample Type Confirmation |
BUB1B is strongly supported by BioGPS gene expression data to be expressed in HEK293 |
| Protein Accession # |
NP_001202.4 |
| Gene Symbol |
BUB1B |
| Predicted Species Reactivity |
Human |
| Application |
WB |
| Predicted Homology Based on Immunogen Sequence |
Human |
| Image 1 | 293, BUB1B
 | Western blot analysis of BUB1B (arrow) using rabbit polyclonal BUB1B Antibody (N-term) (Cat.# OAAB17416). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BUB1B gene (Lane 2) (Origene Technologies). BUB1B is strongly supported by BioGPS gene expression data to be expressed in HEK293 |
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