PI4K2B Antibody - C-terminal region (OAAB17394)

• Product Number: OAAB17394
• Name: PI4K2B Antibody - C-terminal region (OAAB17394)
• Molecular Weight: 54744 Da
• Isotype: Ig
• NCBI Gene Id: 55300
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: PIK42B, PI4KIIB
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: PI 4 Kinase type 2 beta antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Together with PI4K2A and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. This contribution may be especially significant in plasma membrane, endosomal and Golgi compartments. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells and is likely to be involved in the regulation of vesicular trafficking
• Application Info: WB: 1:2000
  WB: 1:1000
  IHC-P: 1:50~100
• Immunogen: This PI 4 Kinase type 2 beta antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 305-336 amino acids from the C-terminal region of human PI 4 Kinase type 2 beta.
• Additional Information: Cellular Location: Cytoplasm. Membrane. Note=Mostly cytoplasmic but also found associated with the plasma membrane, the Golgi and endosomes. Compared to PI4K2A, a larger fraction of PI4K2B is cytosolic due to a smaller extent of palmitoylation. Translocates to membranes where it is recruited by PDGF stimulation by a Rac- GTP-dependent mechanism
  Tissue Location: Widely expressed.
• Uniprot ID: Q8TCG2
• Protein Name: Phosphatidylinositol 4-kinase type 2-beta
• Protein Accession #: NP_060793.2
• Gene Symbol: PI4K2B
• Predicted Species Reactivity: Human, Mouse
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Image 1: hepatocarcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
• Image 2: Mouse Brain, Saos-2
  
  Western blot analysis of anti-PI4K II beta Pab (Cat. # OAAB17394) in mouse brain (Lane A) and Saos-2 (Lane B) cell lysate. PI4K II beta (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.