PI4K2A Antibody - N-terminal region (OAAB17392)

• Product Number: OAAB17392
• Name: PI4K2A Antibody - N-terminal region (OAAB17392)
• Molecular Weight: 54022 Da
• Isotype: Ig
• NCBI Gene Id: 55361
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: PI4KII, PIK42A
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: PI4K2A (PI4K II) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Together with PI4K2B and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells (By similarity)
• Application Info: WB: 1:8000
  WB: 1:1000
  IHC-P: 1:50~100
  IF: 1:10~50
• Immunogen: This PI4K2A (PI4K II) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PI4K2A (PI4K II).
• Additional Information: Cellular Location: Cytoplasm. Membrane (By similarity). Membrane raft. Cell projection, dendrite (By similarity). Cell junction, synapse, presynaptic cell membrane (By similarity). Cell junction, synapse, synaptosome (By similarity). Mitochondrion (By similarity). Endosome (By similarity). Cytoplasmic vesicle (By similarity). Note=Localized in neuronal cell body. Transported from neuronal cell body to neuron project
  Tissue Location: Widely expressed. Highest expression is observed in kidney, brain, heart, skeletal muscle, and placenta and lowest expression is observed in colon, thymus, and small intestine
• Uniprot ID: Q9BTU6
• Protein Name: Phosphatidylinositol 4-kinase type 2-alpha
• Protein Accession #: NP_060895.1
• Gene Symbol: PI4K2A
• Predicted Species Reactivity: Human
• Application: WB, IHC, IF
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: hepatocarcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
• Image 2: Hela
  
  Negative control of hela cells without Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L).Alexa-Fluor-546 emits orange fluorescence. Immunofluorescence analysis of PI4K2A (PI4K II) Antibody (N-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L).
• Image 3: HL-60
  
  Western blot analysis of anti-PI4K II Pab (Cat. # OAAB17392) in HL60 cell lysate. PI4K II (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
• Image 4: Mouse Brain
  
  Western blot analysis of anti-PI4K2A Antibody (N-term D2) in mouse brain tissue lysates (35ug/lane). PI4KII(arrow) was detected using the purified Pab.