PIK3CB Antibody - N-terminal region (Phospho-S139) (OAAB17378)

• Product Number: OAAB17378
• Name: PIK3CB Antibody - N-terminal region (Phospho-S139) (OAAB17378)
• Molecular Weight: 122762 Da
• Isotype: Ig
• NCBI Gene Id: 5291
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: PI3K, PIK3C1, P110BETA, PI3KBETA
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: PIK3CB Antibody (N-term S139) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4- phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5- bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in the activation of AKT1 upon stimulation by G-protein coupled receptors (GPCRs) ligands such as CXCL12, sphingosine 1-phosphate, and lysophosphatidic acid. May also act downstream receptor tyrosine kinases. Required in different signaling pathways for stable platelet adhesion and aggregation. Plays a role in platelet activation signaling triggered by GPCRs, alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) and ITAM (immunoreceptor tyrosine-based activation motif)-bearing receptors such as GP6. Regulates the strength of adhesion of ITGA2B/ ITGB3 activated receptors necessary for the cellular transmission of contractile forces Required for platelet aggregation induced by F2 (thrombin) and thromboxane A2 (TXA2). Has a role in cell survival. May have a role in cell migration. Involved in the early stage of autophagosome formation. Modulates the intracellular level of PtdIns3P (Phosphatidylinositol 3-phosphate) and activates PIK3C3 kinase activity. May act as a scaffold, independently of its lipid kinase activity to positively regulate autophagy. May have a role in insulin signaling as scaffolding protein in which the lipid kinase activity is not required. May have a kinase-independent function in regulating cell proliferation and in clathrin-mediated endocytosis. Mediator of oncogenic signal in cell lines lacking PTEN. The lipid kinase activity is necessary for its role in oncogenic transformation. Required for the growth of ERBB2 and RAS driven tumors
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
• Immunogen: This PIK3CB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124-154 amino acids from the N-terminal region of human PIK3CB.
• Additional Information: Cellular Location: Cytoplasm. Nucleus. Note=Interaction with PIK3R2 is required for nuclear localization and export
  Tissue Location: Expressed ubiquitously.
• Uniprot ID: P42338
• Protein Name: Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform
• Sample Type Confirmation:

  PIK3CB is strongly supported by BioGPS gene expression data to be expressed in MDA-MB435

• Protein Accession #: NP_006210.1
• Gene Symbol: PIK3CB
• Predicted Species Reactivity: Human
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: Human Pancreas
  
  PIK3CB Antibody (N-term S139) (Cat. # OAAB17378) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PIK3CB Antibody (N-term S139) for immunohistochemistry. Clinical relevance has not been evaluated.
• Image 2: MB435, MDA-MB435
  
  PIK3CB Antibody (N-term S139) (Cat. # OAAB17378) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the PIK3CB antibody detected the PIK3CB protein (arrow).
• Image 3: 293
  
  Western blot analysis of PIK3CB (arrow) using rabbit polyclonal PIK3CB Antibody (N-term S139) (Cat. # OAAB17378). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PIK3CB gene.

  PIK3CB is strongly supported by BioGPS gene expression data to be expressed in MDA-MB435