PI3KC3 Antibody - N-terminal region (OAAB17375)

• Product Number: OAAB17375
• Name: PI3KC3 Antibody - N-terminal region (OAAB17375)
• Molecular Weight: 101549 Da
• Isotype: Ig
• NCBI Gene Id: 5289
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: VPS34, Vps34, hVps34
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: PI3KC3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes. Involved in the transport of lysosomal enzyme precursors to lysosomes Required for the abcission step in cytokinesis. Required for transport from early to late endosomes
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  IF: 1:10~50
• Immunogen: This PI3KC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-53 amino acids from the N-terminal region of human PI3KC3.
• Additional Information: Cellular Location: Midbody. Late endosome.
  Tissue Location: Ubiquitously expressed, with a highest expression in skeletal muscle
• Uniprot ID: Q8NEB9
• Protein Name: Phosphatidylinositol 3-kinase catalytic subunit type 3
• Protein Accession #: NP_002638.2
• Gene Symbol: PIK3C3
• Predicted Species Reactivity: Human
• Application: WB, IHC, IF
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: Hela
  
  Western blot analysis of anti-PI3KC3 Pab (Cat. # OAAB17375) in HeLa cell lysate. PI3KC3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
• Image 2: hepatocarcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
• Image 3: Hela, Nuclear
  
  Confocal immunofluorescent analysis of PI3KC3 Antibody (N-term) (Cat# OAAB17375) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).