ARH Antibody - C-terminal region (OAAB17374)

• Product Number: OAAB17374
• Name: ARH Antibody - C-terminal region (OAAB17374)
• Molecular Weight: 33885 Da
• Isotype: Ig
• NCBI Gene Id: 26119
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: ARH, ARH1, ARH2, FHCB1, FHCB2, FHCL4
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: ARH (LDLRAP1) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Adapter protein (clathrin-associated sorting protein (CLASP)) required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts). May be required for LDL binding and internalization but not for receptor clustering in coated pits. May facilitate the endocytocis of LDLR and LDLR-LDL complexes from coated pits by stabilizing the interaction between the receptor and the structural components of the pits. May also be involved in the internalization of other LDLR family members Binds to phosphoinositides, which regulate clathrin bud assembly at the cell surface
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
• Immunogen: This ARH (LDLRAP1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 277-306 amino acids from the C-terminal region of human ARH (LDLRAP1).
• Additional Information: Cellular Location: Cytoplasm.
  Tissue Location: Expressed at high levels in the kidney, liver, and placenta, with lower levels detectable in brain, heart, muscle, colon, spleen, intestine, lung, and leukocytes
• Uniprot ID: Q5SW96
• Protein Name: Low density lipoprotein receptor adapter protein 1
• Protein Accession #: NP_056442.2
• Gene Symbol: LDLRAP1
• Predicted Species Reactivity: Human
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: Human hepatocarcinoma
  
  Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with LDLRAP1 antibody (C-term) (Cat.# OAAB17374), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
• Image 2: NCI-H460
  
  Western blot analysis of LDLRAP1 Antibody (C-term) (Cat.# OAAB17374) in NCI-H460 cell line lysates (35ug/lane). LDLRAP1 (arrow) was detected using the purified Pab (1:60 dilution).